Centrosomes and spindle pole bodies [electronic resource] / edited by Robert E. Palazzo, Trisha N. Davis.

Includes bibliographical references and index.

Purification and reconstitution of Drosophila [gamma]-tubulin complexes Ruwanthi N. Gunawardane [and others] -- Using rapid freeze and freeze-substitution for the preparation of yeast cells for electron microscopy and three-dimensional analysis / Thomas H. Giddings, Jr. [and others] -- Digital fluorescence microscopy of cell cytoplasts with and without the centrosome / Vladimir Rodionov [and others] -- Methods for the study of pericentrin in centrosome assembly and function / Aruna Purohit, German A. Pihan, and Stephen J. Doxsey -- Molecular dissection of yeast spindle pole bodies by two hybrid, in vitro binding, and co-purification / C. Schramm, C. Janke, and E. Schiebel -- Genetic analysis of yeast spindle pole bodies / Trisha N. Davis -- Methods for the study of centrosomes in Drosophila during embryogenesis / Uyen Tram [and others] -- Methods for identification of centrosome-associated proteins / Ryoko Kuriyama [and others] -- Reconstitution of centrosome microtubule nucleation in Drosophila / Michelle Moritz [and others] -- Reconstitution of centrosome microtubule nucleation in Spisula / Bradley J. Schnackenberg and Robert E. Palazzo -- In vitro approaches for the study of microtubule nucleation at the fission yeast spindle pole body / Hirohisa Masuda [and others] -- Gamma tubulin and microtubule nucleation in mammalian cells / Harish C. Joshi and Jun Zhou -- Gamma tubulin in plant cells / Yulia Ovechkina and Berl R. Oakley -- Centrosomes and parthenogenesis / Fr�ed�eric Tournier and Michel Bornens -- In vitro approaches for the study of molecular motors in aster formation / Duane A. Compton -- Methods for the study of centrosome-independent spindle assembly in Xenopus extracts / Sarah M. Wignall and Rebecca Healt -- Methods for the study of centrosome reproduction in mammalian cells / Ron Balczon -- Centrosome reproduction in Xenopus lysates / Edward H. Hinchcliffe and Greenfield Sluder -- Centrosome reproduction in vitro: mammalian centrosomes in Xenopus lysates / Matthieu Piel and Michel Bornens -- Identification of centrosome kinases / Andrew M. Fry and Alison J. Faragher -- Methods for the analysis of centrosome reproduction in cancer cells / Wilma L. Lingle and Jeffrey L. Salisbury -- Isolation of centrosomes from Dictyostelium / Ralph Gr�af.

Containing a comprehensive collection of convenient and quantitative methods for studying centrosomes, spindle pole bodies and related organelles, this text is a valuable resource for researchers and others interested in studying the role of these organelles in cell replication. Chapters outlining the role of these organelles in other cell functions are also included, and a wide variety of experimental systems for analyzing these organelles are presented. Detailed protocols for experiments are contained in each chapter for researchers to perform in their own labs. This volume outlines key meth.

Print version record.