Guide to techniques in mouse development. Part B, Mouse molecular genetics [electronic resource] / edited by Paul M. Wassarman and Philippe M. Soriano.

Originally published in one volume: Guide to techniques in mouse development / Paul M. Wassarman, Melvin L. DePamphilis. San Diego : Academic Press, �1993.

Includes bibliographical references and indexes.

Part B. Lentivirus transgenesis -- Lentivirus transgenesis -- Germline modification using mouse spermatogonial stem cells -- Embryonic in vivo electroporation in the mouse -- Current applications of transposons in mouse genetics -- Functional genomics in the mouse using the sleeping beauty transposon system -- The use of DNA transposons for cancer gene discovery in mice -- A practical summary of site-specific recombination, conditional mutagenesis, and tamoxifen induction of creert2 -- A recombineering pipeline to make conditional targeting constructs -- Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria -- Genetic fate mapping using site-specific recombinases -- Mapping cell fate and function using recombinase-based intersectional strategies -- Genome-wide forward genetic screens in mouse es cells -- Gene trap mutagenesis in the mouse -- A wider context for gene trap mutagenesis -- Mouse mutagenesis with the chemical supermutagen enu -- Phenotype-driven mouse enu mutagenesis screens -- Using enu mutagenesis for phenotype-driven analysis of the mouse -- Exploration of self-renewal and pluripotency in es cells using rnai -- Transgenic rnai applications in the mouse -- Gene knockdown in the mouse through rnai -- In vivo analysis of gene knockdown in tetracycline-inducible shrna mice -- The power of reversibility regulating gene activities via tetracycline-controlled transcription -- Gene expression profiling of mouse oocytes and preimplantation embryos -- Interrogating the transcriptome of oocytes and preimplantation embryos -- Gene expression profiling of mouse embryos with microarrays -- Technological advances in mouse development studies.

This volume�comprehensively covers�new technologies�and methodologies that have appeared for the study of mouse development.�� This volume is Part B�of an update�of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993.� Comprehensively covers�new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap.

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