Guide to techniques in mouse development.
Guide to techniques in mouse development. Part B, Mouse molecular genetics [electronic resource] /
Mouse molecular genetics
edited by Paul M. Wassarman and Philippe M. Soriano.
- 1st ed.
- Amsterdam ; Boston : Elsevier /Academic Press, 2010.
- 1 online resource (xlviii, 580 pages, [8] pages of plates) : illustrations (some color).
- Methods of enzymology, v. 477 0076-6879 ; .
- Methods in enzymology ; v. 477. .
Originally published in one volume: Guide to techniques in mouse development / Paul M. Wassarman, Melvin L. DePamphilis. San Diego : Academic Press, �1993.
Includes bibliographical references and indexes.
Part B. Lentivirus transgenesis -- Lentivirus transgenesis -- Germline modification using mouse spermatogonial stem cells -- Embryonic in vivo electroporation in the mouse -- Current applications of transposons in mouse genetics -- Functional genomics in the mouse using the sleeping beauty transposon system -- The use of DNA transposons for cancer gene discovery in mice -- A practical summary of site-specific recombination, conditional mutagenesis, and tamoxifen induction of creert2 -- A recombineering pipeline to make conditional targeting constructs -- Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria -- Genetic fate mapping using site-specific recombinases -- Mapping cell fate and function using recombinase-based intersectional strategies -- Genome-wide forward genetic screens in mouse es cells -- Gene trap mutagenesis in the mouse -- A wider context for gene trap mutagenesis -- Mouse mutagenesis with the chemical supermutagen enu -- Phenotype-driven mouse enu mutagenesis screens -- Using enu mutagenesis for phenotype-driven analysis of the mouse -- Exploration of self-renewal and pluripotency in es cells using rnai -- Transgenic rnai applications in the mouse -- Gene knockdown in the mouse through rnai -- In vivo analysis of gene knockdown in tetracycline-inducible shrna mice -- The power of reversibility regulating gene activities via tetracycline-controlled transcription -- Gene expression profiling of mouse oocytes and preimplantation embryos -- Interrogating the transcriptome of oocytes and preimplantation embryos -- Gene expression profiling of mouse embryos with microarrays -- Technological advances in mouse development studies.
This volume�comprehensively covers�new technologies�and methodologies that have appeared for the study of mouse development.�� This volume is Part B�of an update�of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993.� Comprehensively covers�new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap.
9780123848819 (electronic bk.) 0123848814 (electronic bk.) 9780123848802 (electronic bk.) 0123848806 (electronic bk.)
Mice--Cytology.
Mice--Embryology.
Mice--Development.
Mice--growth & development--Laboratory Manuals.
Mice--embryology--Laboratory Manuals.
Cell Line--Laboratory Manuals.
Embryonic Stem Cells--cytology--Laboratory Manuals.
Medicine.
SCIENCE--Life Sciences--Cell Biology.
Electronic books.
QP601 / .M49 v. 477eb SF407.M5 / G85 2010eb
571.61935
Originally published in one volume: Guide to techniques in mouse development / Paul M. Wassarman, Melvin L. DePamphilis. San Diego : Academic Press, �1993.
Includes bibliographical references and indexes.
Part B. Lentivirus transgenesis -- Lentivirus transgenesis -- Germline modification using mouse spermatogonial stem cells -- Embryonic in vivo electroporation in the mouse -- Current applications of transposons in mouse genetics -- Functional genomics in the mouse using the sleeping beauty transposon system -- The use of DNA transposons for cancer gene discovery in mice -- A practical summary of site-specific recombination, conditional mutagenesis, and tamoxifen induction of creert2 -- A recombineering pipeline to make conditional targeting constructs -- Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria -- Genetic fate mapping using site-specific recombinases -- Mapping cell fate and function using recombinase-based intersectional strategies -- Genome-wide forward genetic screens in mouse es cells -- Gene trap mutagenesis in the mouse -- A wider context for gene trap mutagenesis -- Mouse mutagenesis with the chemical supermutagen enu -- Phenotype-driven mouse enu mutagenesis screens -- Using enu mutagenesis for phenotype-driven analysis of the mouse -- Exploration of self-renewal and pluripotency in es cells using rnai -- Transgenic rnai applications in the mouse -- Gene knockdown in the mouse through rnai -- In vivo analysis of gene knockdown in tetracycline-inducible shrna mice -- The power of reversibility regulating gene activities via tetracycline-controlled transcription -- Gene expression profiling of mouse oocytes and preimplantation embryos -- Interrogating the transcriptome of oocytes and preimplantation embryos -- Gene expression profiling of mouse embryos with microarrays -- Technological advances in mouse development studies.
This volume�comprehensively covers�new technologies�and methodologies that have appeared for the study of mouse development.�� This volume is Part B�of an update�of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993.� Comprehensively covers�new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap.
9780123848819 (electronic bk.) 0123848814 (electronic bk.) 9780123848802 (electronic bk.) 0123848806 (electronic bk.)
Mice--Cytology.
Mice--Embryology.
Mice--Development.
Mice--growth & development--Laboratory Manuals.
Mice--embryology--Laboratory Manuals.
Cell Line--Laboratory Manuals.
Embryonic Stem Cells--cytology--Laboratory Manuals.
Medicine.
SCIENCE--Life Sciences--Cell Biology.
Electronic books.
QP601 / .M49 v. 477eb SF407.M5 / G85 2010eb
571.61935
