Green fluorescent proteins [electronic resource] / edited by Kevin F. Sullivan, Steve A. Kay.

"Prepared under the auspices of the American Society for Cell Biology."

Includes bibliographical references and index.

Biophysics of the green fluorescent protein / F.G. Prendergast -- Understanding structure-function relationships in the Aequorea victoria green fluorescent protein / Andrew B. Cubitt, Leslie A. Woollenweber, Roger Heim -- Quantitative imaging of the green fluorescent protein (GFP) / David W. Piston, George H. Patterson, Susan M. Knobel -- Single-molecule fluorescence detection of green fluorescence protein and application to single-protein dynamics / Daniel W. Pierce, Ronald D. Vale -- Targeting GFP to organelles / Francesca De Giorgi [and others] -- Cytoskeletal dynamics in yeast / Janet L. Carminati, Tim Stearns -- Analysis of nuclear transport in vivo / Paul Ferrigno, Pamela A. Silver -- GFP fusion proteins as probes for cytology in fission yeast / Kenneth E. Sawin -- GFP variants for multispectral imaging of living cells / James Haseloff -- GFP fusions to a microtubule motor protein to visualize meiotic and mitotic spindle dynamics in Drosophila / Sharyn A. Endow -- GFP as a cell and developmental marker in the Drosophila nervous system / Andrea Brand -- Using time-lapse confocal microscopy for analysis of centromere dynamics in human cells / Kevin F. Sullivan, Richard D. Shelby -- Visualization of large-scale chromatin structure and dynamics using the lac operator/lac repressor reporter system / Andrew S. Belmont [and others] -- Centrosome dynamics in living cells / Aaron Young [and others] -- Transfections of primary muscle cell cultures with plasmids coding for GFP linked to full-length and truncated muscle proteins / Guissou A. Dabiri [and others] -- Monitoring the dynamics and mobility of membrane proteins tagged with green fluorescent protein / J. Lippincott-Schwartz [and others] -- Synchronous real-time reporting of multiple cellular events / Jeffrey D. Plautz, Steve A. Kay -- Visualizing protein interactions in living cells using digitized GFP imaging and FRET microscopy / Ammasi Periasamy, Richard N. Day -- Flow cytometric analysis and FACS sorting of cells based on GFP accumulation / David W. Galbraith, Michael T. Anderson, Leonard A. Herzenberg -- GFP biofluorescence : imaging gene expression and protein dynamics in living cells / Paul C. Goodwin.

This volume is an authoritative and comprehensive treatment of the approaches and techniques used for Green Fluorescent Proteins (GFP). The primary focus of this work is on research using biological systems. The volume covers all aspects of GFP, from its expression in different organisms to specific microscopic and data analysis methods. Key Features* Only volume on Green Fluorescent Protein research* Covers all aspects of GFP* Provides specific microscopic and data analysis methods* Discusses the design and construction of GFP fusion proteins* Covers GFP expression in animals, insects, plants.

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