Green fluorescent proteins
Green fluorescent proteins [electronic resource] /
edited by Kevin F. Sullivan, Steve A. Kay.
- San Diego, Calif. ; London : Academic Press, �1999.
- 1 online resource (xvii, 386 pages, [18] pages of plates) : illustrations (some color).
- Methods in cell biology, v. 58 0091-679X ; .
- Methods in cell biology ; v. 58. .
"Prepared under the auspices of the American Society for Cell Biology."
Includes bibliographical references and index.
Biophysics of the green fluorescent protein / Understanding structure-function relationships in the Aequorea victoria green fluorescent protein / Quantitative imaging of the green fluorescent protein (GFP) / Single-molecule fluorescence detection of green fluorescence protein and application to single-protein dynamics / Targeting GFP to organelles / Cytoskeletal dynamics in yeast / Analysis of nuclear transport in vivo / GFP fusion proteins as probes for cytology in fission yeast / GFP variants for multispectral imaging of living cells / GFP fusions to a microtubule motor protein to visualize meiotic and mitotic spindle dynamics in Drosophila / GFP as a cell and developmental marker in the Drosophila nervous system / Using time-lapse confocal microscopy for analysis of centromere dynamics in human cells / Visualization of large-scale chromatin structure and dynamics using the lac operator/lac repressor reporter system / Centrosome dynamics in living cells / Transfections of primary muscle cell cultures with plasmids coding for GFP linked to full-length and truncated muscle proteins / Monitoring the dynamics and mobility of membrane proteins tagged with green fluorescent protein / Synchronous real-time reporting of multiple cellular events / Visualizing protein interactions in living cells using digitized GFP imaging and FRET microscopy / Flow cytometric analysis and FACS sorting of cells based on GFP accumulation / GFP biofluorescence : imaging gene expression and protein dynamics in living cells / F.G. Prendergast -- Andrew B. Cubitt, Leslie A. Woollenweber, Roger Heim -- David W. Piston, George H. Patterson, Susan M. Knobel -- Daniel W. Pierce, Ronald D. Vale -- Francesca De Giorgi [and others] -- Janet L. Carminati, Tim Stearns -- Paul Ferrigno, Pamela A. Silver -- Kenneth E. Sawin -- James Haseloff -- Sharyn A. Endow -- Andrea Brand -- Kevin F. Sullivan, Richard D. Shelby -- Andrew S. Belmont [and others] -- Aaron Young [and others] -- Guissou A. Dabiri [and others] -- J. Lippincott-Schwartz [and others] -- Jeffrey D. Plautz, Steve A. Kay -- Ammasi Periasamy, Richard N. Day -- David W. Galbraith, Michael T. Anderson, Leonard A. Herzenberg -- Paul C. Goodwin.
This volume is an authoritative and comprehensive treatment of the approaches and techniques used for Green Fluorescent Proteins (GFP). The primary focus of this work is on research using biological systems. The volume covers all aspects of GFP, from its expression in different organisms to specific microscopic and data analysis methods. Key Features* Only volume on Green Fluorescent Protein research* Covers all aspects of GFP* Provides specific microscopic and data analysis methods* Discusses the design and construction of GFP fusion proteins* Covers GFP expression in animals, insects, plants.
9780080859569 (electronic bk.) 0080859569 (electronic bk.) 0125441606 (electronic bk.) 9780125441605 (electronic bk.) 1281755567 9781281755568
Green fluorescent protein.
Luminescent Proteins--analysis.
Luminescent Proteins--genetics.
Luminescent Proteins--chemistry.
Microscopy, Fluorescence.
Prot�eine fluorescente verte.
SCIENCE--Life Sciences--Biochemistry.
Green fluorescent protein.
Eiwitten.
Fluorescentie.
Prote�inas de fluoresc�encia verde.
Prote�inas luminescentes.
Luminesc�encia.
Electronic books.
QH585 / .M47eb vol. 58
572.6
442 / P92 v.58
W1 / ME9615d v.58 1999 QU 55 / G795 1999
"Prepared under the auspices of the American Society for Cell Biology."
Includes bibliographical references and index.
Biophysics of the green fluorescent protein / Understanding structure-function relationships in the Aequorea victoria green fluorescent protein / Quantitative imaging of the green fluorescent protein (GFP) / Single-molecule fluorescence detection of green fluorescence protein and application to single-protein dynamics / Targeting GFP to organelles / Cytoskeletal dynamics in yeast / Analysis of nuclear transport in vivo / GFP fusion proteins as probes for cytology in fission yeast / GFP variants for multispectral imaging of living cells / GFP fusions to a microtubule motor protein to visualize meiotic and mitotic spindle dynamics in Drosophila / GFP as a cell and developmental marker in the Drosophila nervous system / Using time-lapse confocal microscopy for analysis of centromere dynamics in human cells / Visualization of large-scale chromatin structure and dynamics using the lac operator/lac repressor reporter system / Centrosome dynamics in living cells / Transfections of primary muscle cell cultures with plasmids coding for GFP linked to full-length and truncated muscle proteins / Monitoring the dynamics and mobility of membrane proteins tagged with green fluorescent protein / Synchronous real-time reporting of multiple cellular events / Visualizing protein interactions in living cells using digitized GFP imaging and FRET microscopy / Flow cytometric analysis and FACS sorting of cells based on GFP accumulation / GFP biofluorescence : imaging gene expression and protein dynamics in living cells / F.G. Prendergast -- Andrew B. Cubitt, Leslie A. Woollenweber, Roger Heim -- David W. Piston, George H. Patterson, Susan M. Knobel -- Daniel W. Pierce, Ronald D. Vale -- Francesca De Giorgi [and others] -- Janet L. Carminati, Tim Stearns -- Paul Ferrigno, Pamela A. Silver -- Kenneth E. Sawin -- James Haseloff -- Sharyn A. Endow -- Andrea Brand -- Kevin F. Sullivan, Richard D. Shelby -- Andrew S. Belmont [and others] -- Aaron Young [and others] -- Guissou A. Dabiri [and others] -- J. Lippincott-Schwartz [and others] -- Jeffrey D. Plautz, Steve A. Kay -- Ammasi Periasamy, Richard N. Day -- David W. Galbraith, Michael T. Anderson, Leonard A. Herzenberg -- Paul C. Goodwin.
This volume is an authoritative and comprehensive treatment of the approaches and techniques used for Green Fluorescent Proteins (GFP). The primary focus of this work is on research using biological systems. The volume covers all aspects of GFP, from its expression in different organisms to specific microscopic and data analysis methods. Key Features* Only volume on Green Fluorescent Protein research* Covers all aspects of GFP* Provides specific microscopic and data analysis methods* Discusses the design and construction of GFP fusion proteins* Covers GFP expression in animals, insects, plants.
9780080859569 (electronic bk.) 0080859569 (electronic bk.) 0125441606 (electronic bk.) 9780125441605 (electronic bk.) 1281755567 9781281755568
Green fluorescent protein.
Luminescent Proteins--analysis.
Luminescent Proteins--genetics.
Luminescent Proteins--chemistry.
Microscopy, Fluorescence.
Prot�eine fluorescente verte.
SCIENCE--Life Sciences--Biochemistry.
Green fluorescent protein.
Eiwitten.
Fluorescentie.
Prote�inas de fluoresc�encia verde.
Prote�inas luminescentes.
Luminesc�encia.
Electronic books.
QH585 / .M47eb vol. 58
572.6
442 / P92 v.58
W1 / ME9615d v.58 1999 QU 55 / G795 1999
