Mass spectrometry [electronic resource] : modified proteins and glycoconjugates / edited by A.L. Burlingame.

Contributor(s): Burlingame, A. LMaterial type: Text

TextSeries: Methods in enzymology ; v. 405.Publication details: Amsterdam ; Boston : Elsevier Academic Press, c2005. Description: 1 online resource (xxxiii, 432 p., [4] p. of plates) : ill. (some col.)ISBN: 0121828107 (electronic bk.); 9780121828103 (electronic bk.)Subject(s): Mass spectrometry | Biomolecules -- Analysis | Proteins -- Analysis | Glycoconjugates -- Analysis | Glycolipids -- Analysis | Enzymes | Proteins | Mass Spectrometry -- methods | Glycoconjugates -- analysis | Glycolipids -- analysis | Proteins -- analysis | EnzymesGenre/Form: Electronic books.Additional physical formats: Print version:: Mass spectrometry.DDC classification: 547.7 LOC classification: QP601 | .M49 vol.405NLM classification: W1 | QC 454.M3Other classification: 42.03 | 35.79 | 35.27 Online resources: ScienceDirect | ScienceDirect | Volltext

Contents:

Molecular weight determination of peptides and proteins by ESI and MALDI / Kerstin Strupat -- Batch introduction techniques / Connie R. Jim�enez -- In-solution digestion of proteins for mass spectrometry / Katalin F. Medzihradszky -- Identification of phosphorylation sites using microimmobilized metal affinity chromatography / Garry L. Corthals, Reudi Aebersold, and David R. Goodlett -- Mapping posttranslational modifications of proteins by MS-based selective detection : application to phosphoproteomics / Steven A. Carr, Roland S. Annan, and Michael J. Huddleston -- Characterization of protein N-glycosylation / Katalin F. Medzihradszky -- Methods in enzymology : O-glycosylation of proteins / Jasna Peter-Katalini�c -- Analysis of glycosylphosphatidylinositol protein anchors : the prion protein / Michael A. Baldwin -- Comprehensive mass spectrometric analysis of the 20S proteasome complex / Lan Huang and A.L. Burlingame -- The analysis of multiprotein complexes : the yeast and the human spliceosome as case studies / Gitte Neubauer -- The use of mass spectrometry to identify antigens from proteasome processing / Odile Burlet-Schiltz ... [et al.] -- Glycosphingolipid structural analysis and glycosphingolipidomics / Steven B. Levery -- Mapping bacterial glycolipid complexity using capillary electrophoresis and electrospray mass spectrometry / J. Li ... [et al.].

Summary: This volume provides comprehensive treatment of tools and proper usage for the identification of proteins, affinity chromatography and studies the complexity of protein machines and assemblages, assignment of the most common protein posttranslational modifications (phosphorylation and glycosylation) and glycolipidomics. *Part 2 of 2 volumes about Mass Spectrometry *Discusses peptide and protein cleanup and preparation requirements for mass spectrometry *Explains protein enzymic and chemical digestion strategies *Includes case studies of protein assemblages and machines.

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Includes bibliographical references and index.

Description based on print version record.

This volume provides comprehensive treatment of tools and proper usage for the identification of proteins, affinity chromatography and studies the complexity of protein machines and assemblages, assignment of the most common protein posttranslational modifications (phosphorylation and glycosylation) and glycolipidomics. *Part 2 of 2 volumes about Mass Spectrometry *Discusses peptide and protein cleanup and preparation requirements for mass spectrometry *Explains protein enzymic and chemical digestion strategies *Includes case studies of protein assemblages and machines.

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