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Mass spectrometry

Mass spectrometry modified proteins and glycoconjugates / [electronic resource] : edited by A.L. Burlingame. - Amsterdam ; Boston : Elsevier Academic Press, c2005. - 1 online resource (xxxiii, 432 p., [4] p. of plates) : ill. (some col.) - Methods in enzymology, v. 405 0076-6879 ; . - Methods in enzymology ; v. 405. .

Includes bibliographical references and index.

Molecular weight determination of peptides and proteins by ESI and MALDI / Kerstin Strupat -- Batch introduction techniques / Connie R. Jim�enez -- In-solution digestion of proteins for mass spectrometry / Katalin F. Medzihradszky -- Identification of phosphorylation sites using microimmobilized metal affinity chromatography / Garry L. Corthals, Reudi Aebersold, and David R. Goodlett -- Mapping posttranslational modifications of proteins by MS-based selective detection : application to phosphoproteomics / Steven A. Carr, Roland S. Annan, and Michael J. Huddleston -- Characterization of protein N-glycosylation / Katalin F. Medzihradszky -- Methods in enzymology : O-glycosylation of proteins / Jasna Peter-Katalini�c -- Analysis of glycosylphosphatidylinositol protein anchors : the prion protein / Michael A. Baldwin -- Comprehensive mass spectrometric analysis of the 20S proteasome complex / Lan Huang and A.L. Burlingame -- The analysis of multiprotein complexes : the yeast and the human spliceosome as case studies / Gitte Neubauer -- The use of mass spectrometry to identify antigens from proteasome processing / Odile Burlet-Schiltz ... [et al.] -- Glycosphingolipid structural analysis and glycosphingolipidomics / Steven B. Levery -- Mapping bacterial glycolipid complexity using capillary electrophoresis and electrospray mass spectrometry / J. Li ... [et al.].

This volume provides comprehensive treatment of tools and proper usage for the identification of proteins, affinity chromatography and studies the complexity of protein machines and assemblages, assignment of the most common protein posttranslational modifications (phosphorylation and glycosylation) and glycolipidomics. *Part 2 of 2 volumes about Mass Spectrometry *Discusses peptide and protein cleanup and preparation requirements for mass spectrometry *Explains protein enzymic and chemical digestion strategies *Includes case studies of protein assemblages and machines.

0121828107 (electronic bk.) 9780121828103 (electronic bk.)


Mass spectrometry.
Biomolecules--Analysis.
Proteins--Analysis.
Glycoconjugates--Analysis.
Glycolipids--Analysis.
Enzymes.
Proteins.
Mass Spectrometry--methods.
Glycoconjugates--analysis.
Glycolipids--analysis.
Proteins--analysis.
Enzymes.


Electronic books.

QP601 / .M49 vol.405

547.7

QP601 / .M49 v.405

W1 / ME9615K v.405 2005 QC 454.M3 / M415 2005
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