000			05276cam a2200529Ma 4500
001			ocn326630802
003			OCoLC
005			20141103172223.0
006			m o d
007			cr un|---|||||
008			961212s1996 caua ob 001 0 eng d
040			_aSCPER _beng _cCUSER _dOCLCQ _dCOF _dOCLCQ _dAZU _dOPELS _dOCLCQ _dOPELS _dKUK
019			_a56570538
020			_a0121821765 (electronic bk.)
020			_a9780121821760 (electronic bk.)
035			_a(OCoLC)326630802 _z(OCoLC)56570538
050		4	_aQP601 _b.M49 vol. 275
050		4	_aQP605
060		4	_aW1 _bME9615K v.275 1996
060		4	_aQW 165 _bV813 1996
082	0	4	_a574.1925 _220
049			_aTEFA
245	0	0	_aViral polymerases and related proteins _h[electronic resource] / _cedited by Lawrence C. Kuo, David B. Olsen, Steven S. Carroll.
260			_aSan Diego : _bAcademic Press, _cc1996.
300			_a1 online resource (xxx, 658 p.) : _bill.
490	1		_aMethods in enzymology, _x0076-6879 ; _vv. 275
504			_aIncludes bibliographical references and indexes.
505	2		_aSect. 1. Expression, purification, and characterization -- Purification of viral polymerases: general considerations -- Expression, purification, and characterization of the herpes simplex virus type-1 DNA polymerase -- Assays for poliovirus polymerase, 3Dpol, and authentic RNA replication in HeLa S10 extracts -- RNA-dependent RNA polymerase of hepatitis C virus -- Characterization of coronavirus RNA polymerase gene products -- Expression, purification, and characterization of orthomyxovirus: influenza transcriptase -- Expression, purification, and characterization of rhabdovirus polymerase --Expression and purification of retroviral HIV-1 reverse transcriptase -- Expression, purification, and characterization of DNA polymerases involved in papovavirus replication -- Heterologous expression, purification, and characterization of adenovirus DNA polymerase and preterminal protein -- Expression and characterization of hepadnavirus reverse transcriptases -- Expression, purification, and characterization of vaccinia virus-encoded RNA and poly(A)polymerases -- Viral polymerase-associated 5'(arrow)3' exonucleases: expression, purification, and uses -- Sect. 2. Activity assays: kinetic and screening -- Isotopic assays of viral polymerases and related proteins -- Nonisotopic assays of viral polymerases and related proteins -- Catalytic activities associated with retroviral and viral polymerases -- Approaches to high-volume screening assays of viral polymerases and related proteins -- In situ DNA polymerase and RNase H activity gel assays as applied to hepadnavirus particles -- A system to analyze and identify inhibitors of HIV-1 gene regulation using a defective integrated provirus --Sect. 3. Design and analysis of substrates and inhibitors -- Preparation and use of synthetic oligoribonucleotides as tools for study of viral polymerases -- Use of bacteriophage RNA polymerase in RNA synthesis -- Inhibition of viral polymreases by chain-terminating substrates: a kinetic analysis -- Design of nucleoside analog inhibitors of herpes-virus polymerases -- Development of nonucleoside HIV reverse transcriptase inhibitors -- Analysis of inhibition of retroviral reverse transcriptase -- Novel methods of generating specific oligonucleotide inhibitors of viral polymerases -- Sect. 4. Molecular and structural characterizations -- Analyzing the fidelity of reverse transcription and transcription -- Site-directed mutagenic analysis of viral polymerases and related proteins -- Analysis of resistance mutants of viral polymerases -- Author index -- Subject index.
588			_aDescription based on print version record.
520			_aThe critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * Expression, purification, and characterization * Activity assays * Kinetic and screening * Design and analysis of substrates and inhibitors * Molecular and structural characterizations.
650		0	_aTransferases _xAnalysis.
650		0	_aTransferases _xPurification.
650	1	2	_aDNA, Viral _xphysiology.
650	2	2	_aDNA-Directed DNA Polymerase _xisolation & purification.
650	2	2	_aDNA-Directed RNA Polymerases _xisolation & purification.
653	0		_aEnzymes _aRelated to _aViruses
655		4	_aElectronic books.
700	1		_aKuo, Lawrence C.
700	1		_aOlsen, David B.
700	1		_aCarroll, Steven S.
776	0	8	_iPrint version: _tViral polymerases and related proteins. _dSan Diego : Academic Press, c1996 _z0121821765 _w(OCoLC)36083967
830		0	_aMethods in enzymology ; _vv. 275. _x0076-6879
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/book/9780121821760
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/bookseries/00766879/275
942			_cEB
994			_aC0 _bTEF
999			_c21649 _d21649