000			06748cam a2200649Ia 4500
001			ocn388340291
003			OCoLC
005			20141103172222.0
006			m o d
007			cr cnu---unuuu
008			090611s2008 cauaf ob 001 0 eng d
040			_aN$T _beng _cN$T _dOCLCQ _dCUS _dIDEBK _dE7B _dOCLCQ _dOPELS _dOCLCQ _dOPELS _dOCLCF _dKUK
019			_a646802938 _a712982258
020			_a9780080922072 (electronic bk.)
020			_a0080922074 (electronic bk.)
020			_a9780123743787 (electronic bk.)
020			_a0123743788 (electronic bk.)
035			_a(OCoLC)388340291 _z(OCoLC)646802938 _z(OCoLC)712982258
050		4	_aQP601 _b.M49eb v. 448
060		4	_aQU 25 _bR6267 2008
072		7	_aSCI _x049000 _2bisacsh
082	0	4	_a572.88 _222
049			_aTEFA
245	0	0	_aRNA turnover in eukaryotes _h[electronic resource] : _bnucleases, pathways and analysis of mRNA decay / _cedited by Lynne E. Maquat, Megerditch Kiledjian.
260			_aSan Diego, Calif. ; _aLondon : _bAcademic, _cc2008.
300			_a1 online resource (xlviii, 607 p., [5] p. of plates) : _bill. (some col.)
490	1		_aMethods in enzymology, _x0076-6879 ; _vv. 448
504			_aIncludes bibliographical references and indexes.
505	0		_aAnalysis of mRNA decapping / S.W. Liu ... [et al.] -- A kinetic assay to monitor RNA decapping under single- turnover conditions / B.N. Jones ... [et al.] -- Purification and analysis of the decapping activator Lsm1p-7p-Pat1p complex from yeast / S. Tharun -- Reconstitution of recombinant human LSm complexes for biochemical, biophysical, and cell biological studies / B.L. Zaric and C. Kambach -- Regulated deadenylation in vitro / A.C. Goldstrohm, B.A. Hook and M. Wickens -- Cell-free deadenylation assays with Drosophila embryo extracts / M. Jeske and E. Wahle -- Measuring CPEB-mediated cytoplasmic polyadenylation-deadenylation in Xenopus laevis oocytes and egg extracts / J.H. Kim and J.D. Richter -- The preparation and applications of cytoplasmic extracts from mammalian cells for studying aspects of mRNA decay / K.J. Sokoloski, J. Wilusz and C.J. Wilusz -- In vitro assays of 5' to 3'-exoribonuclease activity / O. Pellegrini ... [et al.] -- Reconstitution of RNA exosomes from human and Saccharomyces cerevisiae cloning, expression, purification, and activity assays / J.C. Greimann and C.D. Lima -- Biochemical studies of the mammalian exosome with intact cells / G. Schilders and G.J. Pruijn -- Determining in vivo activity of the yeast cytoplasmic exosome / D. Schaeffer ... [et al.] -- Approaches for studying PMR1 endonuclease-mediated mRNA decay / Y. Otsuka and D.R. Schoenberg -- Methods to determine mRNA half-life in Saccharomyces cerevisiae / J. Coller -- mRNA Decay analysis in Drosophila melanogaster drug-induced changes in glutathione S-transferase D21 mRNA stability / B. Akgul and C.P. Tu -- Measuring mRNA stability during early Drosophila embryogenesis / J.L. Semotok ... [et al.] -- Messenger RNA half-life measurements in mammalian cells / C.Y. Chen, N. Ezzeddine and A.B. Shyu -- Trypanosomes as a model to investigate mRNA decay pathways / S. Archer ... [et al.] -- Cell type-specific analysis of mRNA synthesis and decay in vivo with uracil phosphoribosyltransferase and 4-thiouracil / M.D. Cleary -- Analysis of cytoplasmic mRNA decay in Saccharomyces cerevisiae / D.O. Passos and R. Parker -- Transcriptome targets of the exosome complex in plants / D. Belostotsky -- Sensitive detection of mRNA decay products by use of reverse-ligation-mediated PCR (RL-PCR) / T. Grange -- Tethering assays to investigate nonsense-mediated mRNA decay activating proteins / N.H. Gehring, M.W. Hentze and A.E. Kulozik -- Assays for determining poly(A) tail length and the polarity of mRNA decay in mammalian cells / E.L. Murray and D.R. Schoenberg -- Analyzing P-bodies in Saccharomyces cerevisiae / T. Nissan and R. Parker -- Real-time and quantitative imaging of mammalian stress granules and processing bodies / N. Kedersha ... [et al.] -- Cell biology of mRNA decay / D. Grunwald, R.H. Singer and K. Czaplinski.
588			_aDescription based on print version record.
520			_aSpecific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements, responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. The control of biological processes, such as cellular growth and differentiation, is dependent on how the genetic material within a cell is expressed. The cellular physiology of mRNA-including mRNA processing, transport, localization, and turnover-is central to the process of gene expression. Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway. Expert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover which are central to the process of gene expression. Offers step-by-step lab instructions including necessary equipment and reagents.
650		0	_aMessenger RNA.
650		0	_aGenetic regulation.
650		0	_aEukaryotic cells.
650		0	_aNucleases.
650	1	2	_aRNA Precursors _vLaboratory Manuals.
650	1	2	_aRNA Stability _xphysiology _vLaboratory Manuals.
650	2	2	_aEukaryotic Cells _xmetabolism _vLaboratory Manuals.
650	2	2	_aRNA, Messenger _xmetabolism _vLaboratory Manuals.
650	2	2	_amRNA Cleavage and Polyadenylation Factors _vLaboratory Manuals.
650		7	_aSCIENCE _xLife Sciences _xMolecular Biology. _2bisacsh
650		7	_aEukaryotic cells. _2fast _0(OCoLC)fst00916456
650		7	_aGenetic regulation. _2fast _0(OCoLC)fst00940086
650		7	_aMessenger RNA. _2fast _0(OCoLC)fst01017394
650		7	_aNucleases. _2fast _0(OCoLC)fst01041058
655		4	_aElectronic books.
700	1		_aMaquat, Lynne.
700	1		_aKiledjian, Megerditch.
776	0	8	_iPrint version: _tRNA turnover in eukaryotes. _dSan Diego, Calif. ; London : Academic, c2008 _z9780123743787 _z0123743788 _w(OCoLC)228364730
830		0	_aMethods in enzymology ; _vv. 448.
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/book/9780123743787
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/bookseries/00766879/448
938			_aebrary _bEBRY _nebr10282237
938			_aEBSCOhost _bEBSC _n272195
942			_cEB
994			_aC0 _bTEF
999			_c21611 _d21611