000			06798cam a2200577Ka 4500
001			ocn682508256
003			OCoLC
005			20141103172222.0
006			m o d
007			cr bn||||||abp
007			cr bn||||||ada
008			101121s1989 caua ob 001 0 eng d
040			_aOCLCE _beng _cOCLCE _dOCLCQ _dCOF _dOPELS _dOCLCQ _dKIJ _dOPELS _dVVL _dKUK _dOCLCA
019			_a846753095
020			_a0121820815 (electronic bk.)
020			_a9780121820817 (electronic bk.)
035			_a(OCoLC)682508256 _z(OCoLC)846753095
042			_adlr
050		4	_aQP601 _b.M49 v.180
060		4	_aQU 58 R627 1989
082	0	4	_a572.8/8 _223
049			_aTEFA
245	0	0	_aRNA processing. _nPart A, _pGeneral methods _h[electronic resource] / _cedited by James E. Dahlberg, John N. Abelson.
260			_aSan Diego : _bAcademic Press, _cc1989.
300			_a1 online resource (xxix, 600 p.) : _bill.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
490	1		_aMethods in enzymology ; _vv. 180
504			_aIncludes bibliographical references.
505	0	0	_tIsolation of RNA / _rShelby L. Berger ... [et al.] -- _tPurification of RNA molecules gel techniques / _rToshimichi Ikemura, James E. Dahlberg and John N. Abelson -- _tPurifying RNA by column chromatography / _rN. Kyle Tanner, James E. Dahlberg and John N. Abelson -- _tSynthesis of long, capped transcripts in Vitro by SP6 and T7 RNA polymerases / _rJoel K. Yisraeli ... [et al.] -- _tSynthesis of small RNAs using T7 RNA polymerase / _rJohn F. Milligan ... [et al.] -- _tIn vitro synthesis of end-mature, intron-containing transfer RNAs / _rVicente M. Reyes ... [et al.] -- _tPreparation of precursors to mRNA from mammalian cell nuclei / _rSelina Chen-Kiang ... [et al.] -- _tPulse labeling of heterogeneous nuclear RNA in isolated nuclei / _rSelina Chen-Kiang ... [et al.] -- _tIsolation and characterization of yeast ribosomal RNA precursors and preribosomes / _rJacobus Klootwuk ... [et al.] -- _tPreparation of yeast transfer RNA precursors in Vivo / _rGayle Knapp, James E. Dahlberg and John N. Abelson -- _tDideoxy sequencing of RNA using reverse transcriptase / _rChang Shahn ... [et al.] -- _tRNA fingerprinting / _rAndrea D. Branch ... [et al.] -- _tEnzymatic RNA sequencing / _rY. Kuchino ... [et al.] -- _tCharacterization of cap structures / _rYasuhiro Furuichi ... [et al.] -- _tIsolation and characterization of branched oligonucleotides from RNA / _rJ. David Reilly ... [et al.] -- _tEnzymatic approaches to probing of RNA secondary and tertiary structure / _rGayle Knapp, James E. Dahlberg and John N. Abelson.
505	0	0	_tA guide for probing native small nuclear RNA and ribonucleoprotein structures / _rAlain Krol ... [et al.] -- _tPhylogenetic comparative analysis of RNA secondary structure / _rBryan D. James ... [et al.] -- _tMapping the genetic organization of RNA by electron microscopy / _rLouise T. Chow ... [et al.] -- _tComputer prediction of RNA structure / _rMichael Zuker, James E. Dahlberg and John N. Abelson -- _tRNA pseudoknot: Structure, detection, and prediction / _rC.W.A. Pleij ... [et al.] -- _tabsorbance melting curves of RNA / _rJoseph D. Puglisi ... [et al.] -- _tNuclease digestion: A method for mapping introns / _rShelby L. Berger, James E. Dahlberg and John N. Abelson -- _tCharacterization of RNA molecules by S1 nuclease analysis / _rArnold J. Berk, James E. Dahlberg and John N. Abelson -- _tPrimer extension analysis of RNA / _rWilliam R. Boorstein ... [et al.] -- _tModification interference analysis of reactions using RNA substrates / _rLaura Conway ... [et al.] -- _tPhotoaffinity cross-linking methods for studying RNA-protein interactions / _rMichelle M. Hanna, James E. Dahlberg and John N. Abelson -- _tUltraviolet-induced cross-linking of rna to proteins in vivo / _rSeraf�in Pinol-Roma ... [et al.] -- _tAnalysis of ultraviolet-induced RNA-RNA cross-links: A means for probing RNA structure-function relationships / _rAndrea D. Branch ... [et al.] -- _tanalysis of splicing complexes and small nuclear ribonucleoprotein particles by native gel electrophoresis / _rMaria M. Konarska, James E. Dahlberg and John N. Abelson -- _tDetermination of RNA-protein and RNA-ribonucleoprotein interactions by nuclease probing / _rKatherine A. Parker ... [et al.] -- _tImmunoprecipitation of ribonucleoproteins using autoantibodies / _rJoan A. Steitz, James E. Dahlberg and John N. Abelson -- _tElectron microscopy of ribonucleoprotein complexes on nascent RNA using miller chromatin spreading method / _rYvonne N. Osheim ... [et al.] -- _tGenetic Methods for identification and characterization of RNA-RNA and RNA-protein interactions / _rRoy Parker, James E. Dahlberg and John N. Abelson -- _tCompilation of small nuclear RNA sequences / _rRam Reddy, James E. Dahlberg and John N. Abelson -- _tSequences and classification of group I and group II introns / _rJohn M. Burke, James E. Dahlberg and John N. Abelson -- _tCompilation of self-cleaving sequences from plant virus satellite RNAs and other sources / _rGeorge Bruening, James E. Dahlberg and John N. Abelson.
506			_3Use copy _fRestrictions unspecified _2star _5MiAaHDL
533			_aElectronic reproduction. _b[S.l.] : _cHathiTrust Digital Library, _d2010. _5MiAaHDL
538			_aMaster and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. _uhttp://purl.oclc.org/DLF/benchrepro0212 _5MiAaHDL
583	1		_adigitized _c2010 _hHathiTrust Digital Library _lcommitted to preserve _2pda _5MiAaHDL
588			_aDescription based on print version record.
520			_aMany methods have been developed or adapted for the study of RNA processing, which plays a key role in the expression of genetic information. Volumes 180 and 181 of Methods in Enzymology, RNA Processing, Parts A and B, represent the first comprehensive collation of protocols used for the preparation of substrates, for the preparation of extracts and enzymes, and for the analysis of products in RNA processing. At least half of the chapters present techniques that are directly applicable to other areas of nucleic acid biochemistry and cell biology.
650		0	_aRNA _xMethodology.
650		6	_aARN.
650		2	_aRNA.
655		4	_aElectronic books.
700	1		_aDahlberg, James.
700	1		_aAbelson, John.
776	0	8	_iPrint version: _tRNA processing. Part A, General methods. _dSan Diego : Academic Press, c1989 _w(OCoLC)20764876
830		0	_aMethods in enzymology ; _vv. 180.
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/book/9780121820817
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/bookseries/00766879/180
942			_cEB
994			_aC0 _bTEF
999			_c21607 _d21607