000 05419cam a2200589Ia 4500
001 ocn802058453
003 OCoLC
005 20141103172222.0
006 m o d
007 cr cn|||||||||
008 120711s2012 ne adf ob 001 0 eng d
040 _aE7B
_beng
_epn
_cE7B
_dN$T
_dCOF
_dOCLCQ
_dEBLCP
_dOCLCQ
_dCUS
_dOPELS
_dYDXCP
_dOPELS
_dOCLCF
_dVVL
_dKUK
_dUKDOC
_dOCLCQ
020 _a9780123972293 (electronic bk.)
020 _a0123972299 (electronic bk.)
020 _z9780123965462
029 1 _aAU@
_b000049649585
035 _a(OCoLC)802058453
050 4 _aQP623.5.C36
_bR53 2012eb
060 4 _aQU 141
072 7 _aSCI
_x007000
_2bisacsh
082 0 4 _a572.76
_223
049 _aTEFA
245 0 0 _aRNA helicases
_h[electronic resource] /
_cedited by Eckhard Jankowsky.
250 _a1st ed.
260 _aAmsterdam ;
_aBoston :
_bElsevier/Academic Press,
_c2012.
300 _a1 online resource (li, 525 pages, [10] pages of plates :) :
_billustrations (some color).
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
490 1 _aMethods in enzymology,
_x0076-6879 ;
_vv. 511
504 _aIncludes bibliographical references and indexes.
505 0 _aAnalysis of duplex unwinding by RNA helicases using stopped-flow fluorescence spectroscopy / Andrea Putnam and Eckhard Jankowsky -- Analyzing ATP utilization by DEAD-box RNA helicases using kinetic and equilibrium methods / Michael J. Bradley and Enrique M. De La Cruz -- Oxygen isotopic exchange probes of ATP hydrolysis by RNA helicases / David D. Hackney -- Conformational changes of DEAD-box helicases monitored by single molecule fluorescence resonance energy transfer / Alexandra Z. Andreou and Dagmar Klostermeier -- RNA catalysis as a probe for chaperone activity of DEAD-box helicases / Jeffery P. Potratz and Rick Russell -- Molecular mechanics of RNA translocases / Steve C. Ding and Anna Pyle -- Analysis of helicase-RNA interactions using nucelotide analog interference mapping / Annie Schwartz [and others] -- Crystallization and X-ray structure determination of an RNA-dependent hexameric helicase / Nathan D. Thomsen and James M. Berger -- Structural analysis of RNA helicases with small-angle X-ray scattering / Manja A. Behrens .. [et al.] -- Analysis of cofactor effects on RNA helicases / Crystal Young and Katrin Karbstein -- Analysis of DEAD-box proteins in mRNA export / Ben Montpetit, Markus A. Seeliger, and Karsten Weis -- Biochemical characterization of the RNA helicase UPF1 involved in nonsense-mediated mRNA decay / Francesca Fiorini, Fabien Bonneau, and Herv�e Le Hir -- Identification of RNA helicase target sites by UV cross-linking and analysis of cDNA / Markus T. Bohnsack, David Tollervey, and Sander Granneman -- In vivo approaches to dissecting the function of RNA helicases in eukaryotic ribosome assembly / David C. Rawling and Susan J. Baserga -- Analysis of RNA helicases in P-bodies and stress granules / Angela Hilliker -- DEAD-box RNA helicases as transcription cofactors / Frances V. Fuller-Pace and Samantha M. Nicol -- DEAD-box RNA helicases in gram-postive RNA decay / Peter Redder and Patrick Linder -- RNA helicases in cyanobacteria : biochemical and molecular approaches / George W. Owttrim -- Determination of host RNA helicases activity in viral replication / Amit Sharma and Kathleen Boris-Lawrie -- Inhibitors of translation targeting eukaryotic translation initiation factor 4A / Regina Cencic, Gabriela Galicia-V�azquez, and Jerry Pelletier -- Identification and analysis of inhibitors targeting the hepatitis C virus NS3 helicase / Alicia M. Hanson [and others].
520 _aThis volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases. The contributions in this volume cover the broad scope of methods in the research on these enzymes. Several chapters describe quantitative biophysical and biochemical approaches to study molecular mechanisms and conformational changes of RNA helicases. Further chapters cover structural analysis, examination of co-factor effects on several representative examples, and the analysis of cellular functions of select enzymes. Two chapters outline approaches to the analysis of inhibitors that target RNA helicases. This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases The contributions in this volume cover the broad scope of methods in the research on these enzymes.
650 0 _aCatalytic RNA.
650 2 _aRNA Helicases
_xanalysis.
650 2 _aRNA, Messenger
_xmetabolism.
650 2 _aRNA, Messenger
_xphysiology.
650 7 _aSCIENCE
_xLife Sciences
_xBiochemistry.
_2bisacsh
650 7 _aCatalytic RNA.
_2fast
_0(OCoLC)fst00848887
655 4 _aElectronic books.
700 1 _aJankowsky, Eckhard.
830 0 _aMethods in enzymology ;
_vv. 511.
_x0076-6879
856 4 0 _3ScienceDirect
_uhttp://www.sciencedirect.com/science/book/9780123965462
856 4 0 _3ScienceDirect
_uhttp://www.sciencedirect.com/science/bookseries/00766879/511
938 _a123Library.org
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938 _aebrary
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938 _aEBSCOhost
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938 _aYBP Library Services
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942 _cEB
994 _aC0
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999 _c21600
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