000			05243cam a2200697Ma 4500
001			ocn326626539
003			OCoLC
005			20141103172221.0
006			m o d
007			cr un|---|||||
008			040812s2004 ne af ob 001 0 eng d
040			_aSCPER _beng _cCUSER _dOCLCQ _dN$T _dYDXCP _dMERUC _dE7B _dOCLCQ _dIDEBK _dCOF _dOCLCQ _dAZU _dOPELS _dOCLCQ _dGBVCP _dOPELS _dOCLCF _dDEBBG _dKUK
019			_a56579276 _a166275211 _a170957987 _a441779016 _a648270550
020			_a9780080497259 (electronic bk.)
020			_a008049725X (electronic bk.)
020			_a0121827933 (electronic bk.)
020			_a9780121827939 (electronic bk.)
029	1		_aDEBBG _bBV023126118
029	1		_aGBVCP _b549195114
035			_a(OCoLC)326626539 _z(OCoLC)56579276 _z(OCoLC)166275211 _z(OCoLC)170957987 _z(OCoLC)441779016 _z(OCoLC)648270550
050		4	_aQP601 _b.M49 vol. 388
050		4	_aTP248.65.P76
060		0	_aQU 55
072		7	_aSCI _x007000 _2bisacsh
082	0	4	_a572/.7 _222
049			_aTEFA
245	0	0	_aProtein engineering _h[electronic resource] / _cedited by Dan E. Robertson, Joseph P. Noel.
260			_aAmsterdam ; _aBoston : _bElsevier Academic Press, _cc2004.
300			_a1 online resource (xxxvii, 413 p., [16] p. of plates) : _bill. (some col.)
490	1		_aMethods in enzymology, _x0076-6879 ; _vv. 388
504			_aIncludes bibliographical references and indexes.
505	0		_aSection I. Methodology -- Gene site saturation mutagenesis : a comprehensive mutagenesis approach -- Single-stranded DNA family shuffling -- Module shuffling -- SCHEMA-guided protein recombination -- Staggered extension process in vitro DNA recombination -- Using incremental truncation to create libraries of hybrid enzymes -- Random multirecombinant polymerase chain reaction -- Gene library synthesis by structure-based combinatorial protein engineering -- New enzymes from combinatorial library modules -- Construction of protein fragment complementation libraries using incremental truncation -- Section II. Applications: optimization and screening -- GigaMatrix: a novel ultrahigh throughput protein optimization and discovery platform -- High throughput microplate screens for directed protein evolution -- Periplasmic expression as a basis for whole cell kinetic screening of unnatural enzyme reactivities -- Engineering the thermotolerance and pH optimum of family 11 xylanases by site-directed mutagenesis -- Screening for oxidative resistance -- Consensus-based engineering of protein stability: from intrabodies to thermostable enzymes -- Improving the functional expression of N-Carbamoylase by directed evolution using the green fluorescent protein fusion reporter system -- Section III. Applications: directed evolution of enzymatic function -- Directed evolution of lipases and esterases -- Protein engineering of the cytochrome P450 monooxygenase from bacillus megaterium -- Directed evolution of aldolases -- Changing the enantioselectivity of enzymes by directed evolution -- Control of stereoselectivity in phosphotriesterase -- Section IV. Applications: evolution of biosynthetic pathways -- Manipulation and analysis of polyketide synthases -- Reactions catalyzed by mature and recombinant nonribosomal peptide synthetases -- Engineering carotenoid biosynthetic pathways -- Section V. Devices, antibodies, and vaccines -- Directed evolution of bacteriorhodopsin for device applications -- Engineering antibody affinity by yeast surface display -- A conditionally replicating virus as a novel approach toward an HIV vaccine.
588			_aDescription based on print version record.
520			_aThis MIE volume covers methods for a multitude of topics among which are computational methods, laboratory methods, enzyme optimization, binding proteins/antibodies, and screening technologies.
650		0	_aProtein engineering.
650		0	_aProteins _xSynthesis.
650		0	_aGenetic engineering.
650		0	_aProteins _xStructure-activity relationships.
650		2	_aGenetic Engineering.
650		2	_aProtein Conformation.
650		2	_aProteins _xgenetics.
650		7	_aSCIENCE _xLife Sciences _xBiochemistry. _2bisacsh
650		7	_aGenetic engineering. _2fast _0(OCoLC)fst00940027
650		7	_aProtein engineering. _2fast _0(OCoLC)fst01079685
650		7	_aProteins _xStructure-activity relationships. _2fast _0(OCoLC)fst01079762
650		7	_aProteins _xSynthesis. _2fast _0(OCoLC)fst01079764
655		4	_aElectronic books.
700	1		_aRobertson, Dan E.
700	1		_aNoel, Joseph P.
776	0	8	_iPrint version: _tProtein engineering. _dAmsterdam ; Boston : Elsevier Academic Press, c2004 _z0121827933 _w(OCoLC)56137371
830		0	_aMethods in enzymology ; _vv. 388. _x0076-6879
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/book/9780121827939
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/bookseries/00766879/388
856	4		_uhttp://www.sciencedirect.com/science/bookseries/00766879 _3Volltext
938			_aYBP Library Services _bYANK _n2609397
938			_aebrary _bEBRY _nebr10180549
938			_aIngram Digital eBook Collection _bIDEB _n101154
938			_aEBSCOhost _bEBSC _n195423
942			_cEB
994			_aC0 _bTEF
999			_c21548 _d21548