000			04787cam a2200589Ia 4500
001			ocn174041933
003			OCoLC
005			20141103172220.0
006			m o d
007			cr cnu---unuuu
008			071011s2007 cauaf ob 001 0 eng d
040			_aN$T _beng _cN$T _dYDXCP _dBTCTA _dOCLCQ _dCUS _dOCLCQ _dUMC _dBAKER _dMBB _dOCLCQ _dOPELS _dOCLCQ _dIAL _dOPELS _dOCLCF _dVVL _dKUK _dOCLCQ
019			_a170994509 _a173240450 _a179791186
020			_a9780080551395 (electronic bk.)
020			_a0080551394 (electronic bk.)
020			_a0123739179 (electronic bk.)
020			_a9780123739179 (electronic bk.)
029	1		_aAU@ _b000042150126
035			_a(OCoLC)174041933 _z(OCoLC)170994509 _z(OCoLC)173240450 _z(OCoLC)179791186
050		4	_aQP601 _b.C733 2007eb
060		4	_aQU 58.7 _bM6258 2007
072		7	_aSCI _x049000 _2bisacsh
082	0	4	_a572.88 _222
049			_aTEFA
245	0	0	_aMicroRNA methods _h[electronic resource] / _cedited by John J. Rossi, Gregory J. Hannon.
260			_aSan Diego, Calif. : _bAcademic Press, _cc2007.
300			_a1 online resource (xl, 261 p., [8] p. of plates) : _bill. (some col.)
490	1		_aMethods in enzymology, _x0076-6879 ; _vv. 427
504			_aIncludes bibliographical references and indexes.
505	0	0	_gCh. 1. _tIdentification of viral microRNAs / _rChristopher S. Sullivan and Adam Grundhoff -- _gCh. 2. _tRobust machine learning algorithms predict microRNA genes and targets / _rPal Saetrom and Ola Snove, Jr. -- _gCh. 3. _tIdenfication of virally encoded microRNAs / _rSebastien Pfeffer -- _gCh. 4. _tComputational methods for microRNA target prediction / _rYuka Watanabe, Masaru Tomita, and Akio Kanai -- _gCh. 5. _tIn vitro and in vivo assays for the activity of Drosha complex / _rYoontae Lee and V. Narry Kim -- _gCh. 6. _tMicroarray analysis of miRNA gene expression / _rJ. Michael Thomson, Joel S. Parker, and Scott M. Hammond -- _gCh. 7. _tCloning and detecting signature microRNAs from mammalian cells / _rGuihua Sun, Haitang Lik and Gohn J. Rossi.
505	8	0	_gCh. 8. _tApproaches for studying microRNA and small interfering RNA methylation in vitro and in vivo / _rZhiyong Yang ... [et al.] -- _gCh. 9. _tAnalysis of small RNA profiles during development / _rToshiaki Watanabe ... [et al.] -- _gCh. 10. _tDissecting microRNA-mediated gene regulation and function in T cell development / _rTin Ky Mao and Chang-Zheng Chen -- _gCh. 11. _tInvestigation of microRNAs alterations in leukemias and lymphomas / _rGeorge Adrian Calin and Carlo Maria Croce -- _gCh. 12. _tDiscovery of pathogen-regulated small RNAs in plants / _rSurekha Katiyar-Agarwal and Hailing Jin -- _gCh. 13. _tProtocols for expression and functional analysis of viral microRNAs / _rEva Gottwein and Bryan R. Cullen.
588			_aDescription based on print version record.
520			_aMicroRNAs (miRNA) are tiny bits of genetic material that were unknown nearly 10 years ago but now represent an exciting field of study in biology. Upon their discovery, researchers revealed for the first time a new mechanism by which microRNA can stop the function of messenger RNA (mRNA) by literally cutting it in half, interfering with the normal function of specific messenger RNAs in gene expression. This "expression" of genes that code for essential proteins is essentially what controls whether a cell turns into a liver, lung, or brain cell, for example. Understanding what activates this process - or stops it - is a key to understanding the biological process and builds a foundation for advances in medicine and other fields. This volume in Methods in Enzymology presents valuable methods for studying MicroRNA, with three sections covering identification of MicroRNAs and their targets; Micro RNA expression, maturation and functional analysis; and MicroRNAs and disease.
650		0	_aSmall interfering RNA.
650		7	_aSCIENCE _xLife Sciences _xMolecular Biology. _2bisacsh
650		7	_aSmall interfering RNA. _2fast _0(OCoLC)fst01121445
650		2	_aMicroRNAs.
650		2	_aRNA, Small Interfering.
655		4	_aElectronic books.
700	1		_aRossi, John J.
700	1		_aHannon, Gregory J., _d1964-
776	0	8	_iPrint version: _tMicroRNA methods. _dSan Diego, Calif. : Academic Press, c2007 _z9780123739179 _z0123739179 _w(OCoLC)156811801
830		0	_aMethods in enzymology ; _vv. 427. _x0076-6879
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/book/9780123739179
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/bookseries/00766879/427
938			_aYBP Library Services _bYANK _n2636994
938			_aBaker and Taylor _bBTCP _nBK0007491353
938			_aBaker & Taylor _bBKTY _c149.95 _d149.95 _i0123739179 _n0007320378 _sactive
938			_aEBSCOhost _bEBSC _n205628
942			_cEB
994			_aC0 _bTEF
999			_c21459 _d21459