000			05597cam a2200601Mi 4500
001			ocn870587054
003			OCoLC
005			20141103172220.0
006			m o d
007			cr cnu---unuuu
008			140215s2014 vtu o 000 0 eng d
040			_aEBLCP _beng _epn _cEBLCP _dN$T _dOPELS _dOCLCF _dOCLCQ _dAZS _dOCLCO
020			_a9780124116061 _q(electronic bk.)
020			_a012411606X _q(electronic bk.)
020			_z9780124116191
035			_a(OCoLC)870587054
050		4	_aQP88.15 _b.M384 2014
072		7	_aSCI _x056000 _2bisacsh
082	0	4	_a571.57
049			_aTEFA
245	0	0	_aMethods of adipose tissue biology. _nPart A _h[electronic resource] / _cedited by Ormond A. Macdougald.
260			_aBurlington : _bElsevier Science, _c2014.
300			_a1 online resource (726 pages)
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
490	0		_aMethods in enzymology ; _vv. 537
588	0		_aPrint version record.
505	0		_aCover image; Title Page; Table of Contents; Copyright; Contributors; Preface; Chapter One: Adipocyte-Specific Transgenic and Knockout Models; Abstract; 1 Introduction; 2 Loss-of-Function Strategies in Adipocytes In Vivo; 3 Gain-of-Function Strategies in Adipocytes In Vivo; Chapter Two: Imaging White Adipose Tissue with Confocal Microscopy; Abstract; 1 Introduction; 2 Materials for Imaging Adipose Tissue; 3 Methods; 4 Summary; Acknowledgment; Chapter Three: Isolation and Study of Adipocyte Precursors; Abstract; 1 Introduction; 2 Adipose Tissue Depots and Cell Populations.
505	8		_a3 Digestion of Whole WAT for Isolation of SVF4 Flow Cytometry and FACS; 5 Primary Adipocyte Precursor Cell Culture and Differentiation; 6 Digestion of Whole WAT for Isolation of Mature Adipocytes; Acknowledgment; Chapter Four: Imaging of Adipose Tissue; Abstract; 1 Introduction; 2 Imaging of Whole Mounted Adipose Tissue; 3 Imaging of Sectioned Adipose Tissue; Acknowledgment; Chapter Five: Adipose Tissue Angiogenesis Assay; Abstract; 1 Introduction; 2 Materials; 3 Methods; 4 Method Limitations; Acknowledgment; Chapter Six: Quantifying Size and Number of Adipocytes in Adipose Tissue; Abstract.
505	8		_a1 Introduction2 Technical Aspects; 3 Future Challenges and Conclusions; Acknowledgment; Chapter Seven: Use of Osmium Tetroxide Staining with Microcomputerized Tomography to Visualize and Quantify Bone Marrow Adipose Tissue In Vivo; Abstract; 1 Introduction; 2 Materials; 3 Methods; 4 Summary; Acknowledgment; Chapter Eight: Brown Adipose Tissue in Humans; Abstract; 1 Introduction; 2 Positron Emission Tomography; 3 Magnetic Resonance Imaging; 4 Dual Energy Computed Tomography; Chapter Nine: Analyzing the Functions and Structure of the Human Lipodystrophy Protein Seipin; Abstract; 1 Introduction.
505	8		_a2 Technical Aspects3 Discussion; Chapter Ten: Differentiation of Human Pluripotent Stem Cells into Highly Functional Classical Brown Adipocytes; Abstract; 1 Introduction; 2 Experimental Components and Considerations; 3 Notes with Troubleshooting; Chapter Eleven: Analysis and Measurement of the Sympathetic and Sensory Innervation of White and Brown Adipose Tissue; Abstract; 1 Introduction; 2 Surgical Denervation of WAT and BAT Nerves; 3 Chemical Denervation of Adipose Tissue Using 6-Hydroxy-Dopamine; 4 Local Sensory Denervation of Adipose Tissue Using Capsaicin.
505	8		_a5 Assessment of Sympathetic Denervation Using NETO or Content as Measured by HPLC-EC6 Assessment of Sensory Denervation Using CGRP ELIA; 7 Expression of Data; Acknowledgment; Chapter Twelve: Measurement and Manipulation of Human Adipose Tissue Blood Flow Using Xenon Washout Technique and Adipose Tissue Microinfusion; Abstract; 1 Introduction; 2 AT 133Xenon Washout-Principle of the Method; 3 AT Microinfusion-Principle of the Method; 4 Materials; 5 Procedure; 6 Calculations; 7 Other Techniques Used in ATBF Measurement; 8 Conclusion; Acknowledgment.
500			_aChapter Thirteen: Isolation and Quantitation of Adiponectin Higher Order Complexes.
520			_aMethods of Adipose Tissue Biology is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers who are world leaders in this field. Detailed lab protocols include methods to visualize adipocytes and adipose tissues in humans and experimental models, converting stem cells into white and brown adipocytes in vitro, evaluating aspects of adipocyte metabolism, inducibly knocking out genes in adipose tissues, and evaluating transcriptional control of adipogenesis on a global scale.
650		0	_aAdipose tissues _vLaboratory manuals.
650		4	_aAdipose tissue _xCytology.
650		4	_aAdipose tissue _xMicrobiology.
650		4	_aAdipose tissues.
650		4	_aCulture techniques _xMethods.
650		7	_aSCIENCE _xLife Sciences _xAnatomy & Physiology. _2bisacsh
650		7	_aAdipose tissues. _2fast _0(OCoLC)fst00796650
655		4	_aElectronic books.
655		7	_aHandbooks, manuals, etc. _2fast _0(OCoLC)fst01423877
700	1		_aMacdougald, Ormond A.
776	0	8	_iPrint version: _aMacdougald, Ormond A. _tMethods of Adipose Tissue Biology Part A. _dBurlington : Elsevier Science, �2014 _z9780124116191
830		0	_aMethods in enzymology ; _vv. 537.
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/book/9780124116191
938			_aEBL - Ebook Library _bEBLB _nEBL1629239
938			_aEBSCOhost _bEBSC _n698427
942			_cEB
994			_aC0 _bTEF
999			_c21454 _d21454