000			03961cam a2200445Ia 4500
001			ocn754850665
003			OCoLC
005			20141103172219.0
006			m o d
007			cr mn|||||||||
008			110927s1997 caua ob 001 0 eng d
040			_aCOF _beng _cCOF _dOPELS _dOCLCQ _dOPELS _dOCLCF _dKUK
020			_a0121821854 (electronic bk.)
020			_a9780121821852 (electronic bk.)
035			_a(OCoLC)754850665
050		4	_aQP601
082	0	4	_a612.01
049			_aTEFA
245	0	0	_aLipases. _nPart A, _pBiotechnology _h[electronic resource] / _cedited by Byron Rubin, Edward A. Dennis.
260			_aSan Diego : _bAcademic Press, _cc1997.
300			_a1 online resource (xxxi, 408 p.) : _bill.
490	1		_aMethods in enzymology ; _vv. 284
504			_aIncludes bibliographical references and indexes.
505	0		_aSect. 1. Primary and tertiary structure -- Structure as a basis for understanding interfacial properties of lipases -- Identification of conserved residues in family of esterase and lipase sequences -- Identification of important motifs in protein sequences: program MULTIM and its applications tolipase-related sequences -- Lipases and [alpha]/[beta] hydrolase fold -- Pancreatic lipases and their complexes with colipases and inhibitors: crystallization and crystal packing -- Impact of structural information on understanding lipolytic function -- Surface and electrostatics of cutinases -- Sect. 2. Isolation, cloning, expression, and engineering -- Site-specific mutagenesis of human pancreatic lipase -- Lipase eingineering: a window into structure-function relationships -- Purification of carboxyl ester lipase (bile salt-stimulated lipase) from human milk and pancreas -- Two novel lipases from thermophile Bacillus thermocatenulatus: screening, purification, cloning, overexpression, and properties -- Vernonia lipase: a plant lipase with strong fatty acid selectivity -- Hepatic lipase: high-level expression and subunit structure determination -- Cloning, sequencing, and expression of Candida rugosa lipases -- Influence of various signal peptides on secretion of mammalian acidic lipases in baculovirus-insect cell system -- Large-scale purification and kinetic properties of recombinant hormone-sensitive lipase from baculovirus-insect cell systems -- New pancreatic lipases: gene expression, protein secretion, and the newborn -- Structure and function of engineered Pseudomonas mendocina lipase -- Protein engineering of microbial lipases of industrial interest -- Glycosylation of bile salt-dependent lipase (cholesterol esterase) -- Steroselectivity of lipase from Rhizopus oryzae toward triaclglycerols and analogs: computer-aided modeling and experimental validation.
588			_aDescription based on print version record.
520			_aThe critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
650		0	_aLipase.
650		0	_aBiotechnology.
650		7	_aBiotechnology. _2fast _0(OCoLC)fst00832729
650		7	_aLipase. _2fast _0(OCoLC)fst00999359
655		4	_aElectronic books.
700	1		_aRubin, Byron.
700	1		_aDennis, Edward A.
776	0	8	_iPrint version: _tLipases. Part A, Biotechnology. _dSan Diego : Academic Press, c1997 _z0121821854 _w(OCoLC)37609759
830		0	_aMethods in enzymology ; _vv. 284.
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/book/9780121821852
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/bookseries/00766879/284
942			_cEB
994			_aC0 _bTEF
999			_c21418 _d21418