000			05542cam a2200469Ia 4500
001			ocm56557299
003			OCoLC
005			20141103172218.0
006			m o d
007			cr |m|n u|||||
008			040922s1991 cau ob 001 0 eng
040			_aAZU _beng _cAZU _dOCLCQ _dOPELS _dOCLCQ _dOPELS _dOCLCF _dKUK
020			_a0121820955 (electronic bk.)
020			_a9780121820954 (electronic bk.)
020			_z0123106702
020			_z9780123106704
035			_a(OCoLC)56557299
050		4	_aQP601
082	0	4	_a589.233 _220
049			_aTEFA
245	0	0	_aGuide to yeast genetics and molecular biology _h[electronic resource] / _cedited by Christine Guthrie, Gerald R. Fink.
260			_aSan Diego : _bAcademic Press, _cc1991.
300			_a1 online resource (xxxvii, 933 p.)
490	1		_aMethods in enzymology ; _vv. 194
500			_aIncludes indexes.
504			_aIncludes bibliographical references.
505	2		_aGetting started with yeast / Fred Sherman -- Micromanipulation and dissection of asci / Fred Sherman, James Hicks -- Mapping yeast genes / Fred Sherman, Paul Wakem -- Positional mapping of genes by chromosome blotting and chromosome fragmentation / Sandra L. Gerring, Carla Connelly, Philip Hieter -- Assay of yeast mating reaction / George F. Sprague, Jr. -- Monitoring meiosis and sporulation in Saccharomyces cerevisiae / Yona Kassir, Giora Simchen -- Identifying sporulation genes, visualizing synaptonemal complexes, and large-scale spore and spore wall purification / Rochelle Easton Esposito, Michael Dresser, Michael Breitenbach -- Putting the HO gene to work : practical uses for mating-type switching / Ira Herskowitz, Robert E. Jensen -- Spore enrichment / Beth Rockmill, Eric J. Lambie, G. Shirleen Roeder -- Analysis and manipulation of yeast mitochondrial genes / Thomas D. Fox ... [et al.] -- DNA of Saccharomyces cerevisiae / Peter Philippsen, Agathe Stotz, Christine Scherf -- High-efficiency transformation of yeast by electroporation / Daniel M. Becker, Leonard Guarente -- Transmission of plasmid DNA to yeast by conjugation with bacteria / Jack A. Heinemann, George F. Sprague, Jr. -- Cloning genes by complementation in yeast / Mark D. Rose, James R. Broach -- Gene isolation with [lambda]gt11 system / Richard A. Young, Ronald W. Davis -- Gene overexpression in studies of Saccharomyces cerevisiae / Jasper Rine -- Preparation of clone libraries in yeast artificial-chromosome vectors / David T. Burke, Maynard V. Olson -- Classical mutagenesis techniques / Christopher W. Lawrence -- Targeting, disruption, replacement, and allele rescue : integrative DNA transformation in yeast / Rodney Rothstein -- In vitro mutagenesis and plasmid shuffling : from cloned gene to mutant yeast / Robert S. Sikorski, Jef D. Boeke -- Recovery of plasmids from yeast into Escherichia coli : shuttle vectors / Jeffrey N. Strathern, David R. Higgins -- Shuttle mutagenesis : bacterial transposons for genetic manipulations in yeast / Merl F. Hoekstra ... [et al.] -- Ty mutagenesis in Saccharomyces cerevisiae / David J. Garfinkel, Jeffrey N. Strathern -- Transformation of yeast directly with synthetic oligonucleotides / Richard P. Moerschell, Goutam Das, Fred Sherman -- Vectors for expression of cloned genes in yeast : regulation, overproduction, and underproduction / Jane C. Schneider, Leonard Guarente -- Vectors for constitutive and inducible gene expression in yeast / Mark Schena, Didier Picard, Keith R. Yamamoto -- Preparation of high molecular weight RNA / Karl K�ohrer, Horst Domdey -- Preparation and analysis of low molecular weight RNAs and small ribonucleoproteins / Jo Ann Wise -- Measurement of mRNA decay rates in Saccharomyces cerevisiae / by Roy Parker ... [et al.] --Labeling of RNA and phosphoproteins in Saccharomyces cerevisiae / Jonathan R. Warner -- Tackling the protease problem in Saccharomyces cerevisiae / Elizabeth W. Jones -- Structural and functional analysis of yeast ribosomal proteins / H.A. Rau�e, W.H. Mager, R.J. Planta.
588			_aDescription based on print version record.
520			_aGuide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Key Features * Basic Methods in Yeast Genetics * Physical and genetic mapping * Making and recovering mutants * Cloning and Recombinant DNA Methods * High-efficiency transformation * Preparation of yeast artificial chromosome vectors * Basic Methods of Cell Biology * Immunomicroscopy * Protein targeting assays * Biochemistry of Gene Expression * Vectors for regulated expression * Isolation of labeled and unlabeled DNA, RNA, and protein.
650		0	_aYeast fungi _xGenetics.
650		0	_aYeast fungi _xCytology.
650		7	_aYeast fungi _xCytology. _2fast _0(OCoLC)fst01182611
650		7	_aYeast fungi _xGenetics. _2fast _0(OCoLC)fst01182614
655		4	_aElectronic books.
700	1		_aGuthrie, Christine.
700	1		_aFink, Gerald R.
830		0	_aMethods in enzymology ; _vv. 194.
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/book/9780121820954
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/bookseries/00766879/194
942			_cEB
994			_aC0 _bTEF
999			_c21353 _d21353