000			06258cam a2200745Ka 4500
001			ocn755005101
003			OCoLC
005			20141103172217.0
006			m o d
007			cr un||||a|a||
008			110929s1994 caua ob 001 0 eng d
040			_aOCLCE _beng _epn _cOCLCE _dCUSER _dAZU _dOPELS _dOCLCQ _dOPELS _dOCLCQ _dKUK
019			_a56561157 _a326626478
020			_a0121821315 (electronic bk.)
020			_a9780121821319 (electronic bk.)
020			_z9780124436657 (pbk. : alk. paper)
020			_z012443665X (pbk. : alk. paper)
035			_a(OCoLC)755005101 _z(OCoLC)56561157 _z(OCoLC)326626478
050		4	_aQP601 _b.M49 v.230
060		4	_aW1 _bME9615K v.230 1994
060		4	_aQU 75 G946 1994
082	0	4	_a574.19245 _220
049			_aTEFA
245	0	0	_aGuide to techniques in glycobiology _h[electronic resource] / _cedited by William J. Lennarz, Gerald W. Hart.
260			_aSan Diego : _bAcademic Press, _c�1994.
300			_a1 online resource (xxvi, 567 pages) : _billustrations.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
490	1		_aMethods in enzymology ; _vv. 230
504			_aIncludes bibliographical references and indexes.
505	0		_aCarbohydrate composition analysis of glycoconjugates by gas-liquid chromatography/mass spectrometry / Roberta K. Merkle and Izabella Poppe -- Metabolic radiolabeling of glycoconjugates / Ajit Varki -- Nonmetabolic radiolabeling and tagging of glycoconjugates / Carl G. Gahmberg and Martti Tovanen -- Enzymatic deglycosylation of aspargine-linked glycans: purification, properties, and specificity of oligosaccharide-cleaving enzymes from Flavobacterium meningosepticum / Anthony L. Tarentino and Thomas H. Plummer, Jr. -- Release of oligosaccharides from glycoproteins by hydrazinolysis / T.P. Patel and R.B. Parekh -- Use of lectins in analysis of glycoconjugates / Richard D. Cummings -- Saccharide linkage analysis using methylation and other techniques / Rudolf Geyer and Hildegard Geyer.
505	0		_a(cont) Mass spectrometry of carbohydrate-containing bipolymers / Anne Dell [and others] -- p1sH nuclear magnetic resonance spectroscopy of carbohydrate chains of glycoproteins / Herman Van Halbeek -- Determination of sialic acids / Gerd Reuter and Roland Schauer -- Size fractionation of oligosaccharides / Akira Kobata -- High-pH anion-exchange chromatography of glycoprotein-derived carbohydrates / Mark R. Hardy and R. Reid Townsend -- High-perfomance liquid chromatography of pyridylaminated saccharides / Sumihiro Hase -- High-perfomance liquid chromatography of oligosaccharides / Jacques U. Baenziger -- High-resolution polyacrylamide gel electrophoresis of fluorophore-labeled reducing saccharides / Peter Jackson.
505	0		_a(cont) Capillary electrophoresis of oligosaccharides / Robert J. Linhardt -- Glycosidases in structural analysis / Gary S. Jacob and Peter Scudder -- Glycosyltransferases in glycobiology / Monica M. Palcic -- Glycosidase inhibitors in study of glycoconjugates / Gur P. Kaushal and Alan D. Elbein -- Synthesis and uses of azido-substituted nucleoside diphosphate sugar photoaffinity analogs / Anna Radominska and Richard R. Drake -- Glycoform analysis of glycoproteins / R.B. Parekh -- Isolation of glycosphingolipids / Ronald L. Schnaar -- Thin-layer chromatography of glycosphingolipids / Ronald L. Schnaar and Leila K. Needham -- Isolation and characterization of proteoglycans / Vincent C. Hascall [and others] -- Structural analysis of glycosylphosphatidylinositol anchors / Anant K. Menon.
505	0		_a(cont) Detection of O-linked N-acetylglucosamine (O-GlcNAc) on cytoplasmic and nuclear proteins / Elizabeth P. Roquemore, Teh-Ying Chou and Gerald W. Hart -- Identification of polysialic acids in glycoconjugates / Jean Ye [and others] -- Neoglycolipids: probes of oligosaccharide structure, antigenicity, and function / Ten Feizi [and others].
520			_aThe critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Key Features * Detailed procedures newly written for this volume * Extensive practical information * Rationale and strategies for carbohydrate chain analysis Among the topics covered: * Release of Oligosaccharides from Glycoproteins by Hydrazinolysis * Mass Spectrometry of Carbohydrate-Containing Biopolymers * High-resolution Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Reducing Saccharides * Capillary Electrophoresis of Oligosaccharides * Synthesis and Uses of Azido-Substituted Nucleoside Diphosphate Sugar Photoaffinity Analogs * Structural Analysis of Glycosylphosphatidylinositol Anchors * Identification of Polysialic Acids in Glycoconjugates.
588			_aDescription based on print version record.
650		0	_aGlycoconjugates _xTechnique.
650		0	_aGlycoproteins _xTechnique.
650	1	2	_aGlycoconjugates _xanalysis.
650	2	2	_aOligosaccharides _xanalysis.
650		7	_aGlycoprot�eines. _2fmesh
650		7	_aGlycoconjugu�es. _2fmesh
650		7	_aBiochimie _xTechnique. _2ram
650		7	_aGlycoconjugu�es. _2ram
650		7	_aGlycoprot�eines. _2ram
650		7	_aGlycosidases. _2ram
650		7	_aOligosaccharides _xAnalyse. _2ram
650	0	7	_aGlykokonjugate. _2swd
650	0	7	_aMethode. _2swd
650	0	7	_aGlykolipide. _2swd
650	0	7	_aGlykoproteine. _2swd
650	0	7	_aAufsatzsammlung. _2swd
650	0	7	_aOligosaccharide. _2swd
650	0	7	_aChemische Analyse. _2swd
655		4	_aElectronic books.
700	1		_aLennarz, William J.
700	1		_aHart, Gerald W.
776	0	8	_iPrint version: _w(OCoLC)29610556
830		0	_aMethods in enzymology ; _vv. 230.
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/book/9780121821319
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/bookseries/00766879/230
942			_cEB
994			_aC0 _bTEF
999			_c21347 _d21347