000			06980cam a2200565Ia 4500
001			ocn463300660
003			OCoLC
005			20141103172217.0
006			m o d
007			cr mnu||||||||
008			091104s2009 caua ob 001 0 eng
040			_aUMC _beng _cUMC _dSCB _dOCM _dBTCTA _dYDXCP _dUKMGB _dCUS _dCRU _dCDX _dE7B _dOCLCQ _dOPELS _dOCLCQ _dOPELS _dOCLCF _dKUK
019			_a318877741
020			_a9780123745361 (electronic bk.)
020			_a0123745365 (electronic bk.)
020			_z9780123749789 (pbk.)
029	1		_aDEBBG _bBV040084208
035			_a(OCoLC)463300660 _z(OCoLC)318877741
050		4	_aQP601 _b.M49 v. 463
060		4	_aW1 _bME9615K v.463 2009
060		4	_aQU 55 _bG9446 2009
070			_aQP601 _b.G85 2009
082	0	4	_a547.7 _222
049			_aTEFA
245	0	0	_aGuide to protein purification _h[electronic resource] / _cedited by Richard R. Burgess, Murray P. Deutscher.
250			_a2nd ed.
260			_aSan Diego, Calif : _bAcademic Press/Elsevier, _cc2009.
300			_a1 online resource (liii, 851 p.) : _bill.
490	1		_aMethods in enzymology, _x0076-6879 ; _vv. 463
504			_aIncludes bibliographical references and indexes.
505	0		_aWhy purify enzymes? / A. Kornberg -- Strategies and considerations for protein purifications / S. Linn -- Use of bioinformatics in planning a protein purification / R.R. Burgess -- Preparing a purification summary table / R.R. Burgess -- Setting up a laboratory / M.P. Deutscher -- Buffers: principles and practice / V.S. Stoll and J.S. Blanchard -- Measurement of enzyme activity / T.K. Harris and M.M. Keshwani -- Quantitation of protein / J.E. Noble and M.J. Bailey -- Concentration of proteins and removal of solutes / D.R. Evans, J.K. Romero and M. Westoby -- Maintaining protein stability / M.P. Deutscher -- Selecting an appropriate method for expressing a recombinant protein / W.H. Brondyk -- Bacterial systems for production of heterologous proteins / S. Zerbs, A.M. Frank and F.R. Collart -- Expression in the yeast Pichia pastoris / J.M. Cregg ... [et al.] -- Baculovirus-insect cell expression systems / D.L. Jarvis -- Recombinant protein production by transient gene transfer into Mammalian cells / S. Geisse and C. Fux -- Tagging for protein expression / A. Malhotra -- Refolding solubilized inclusion body proteins / R.R. Burgess -- Advances in preparation of biological extracts for protein purification / A.C. Grabski -- Isolation of subcellular organelles and structures / U. Michelsen and J. von Hagen -- Protein precipitation techniques / R.R. Burgess -- Affi-Gel Blue for nucleic acid removal and early enrichment of nucleotide binding proteins / M.P. Deutscher -- Ion-exchange chromatography / A. Jungbauer and R. Hahn -- Gel filtration / E. Stellwagen -- Protein chromatography on hydroxyapatite columns / L.J. Cummings, M.A. Snyder and K. Brisack -- Theory and use of hydrophobic interaction chromatography in protein purification applications / J.T. McCue -- Affinity chromatography: general methods / M. Urh, D. Simpson and K. Zhao -- Immobilized-metal affinity chromatography (IMAC): a review / H. Block ... [et al.] -- Identification, production, and use of polyol-responsive monoclonal antibodies for immunoaffinity chromatography / N.E. Thompson ... [et al.] -- One-dimensional gel electrophoresis / D.E. Garfin -- Isoelectric focusing and two-dimensional gel electrophoresis / D.B. Friedman, S. Hoving and R. Westermeier -- Protein gel staining methods: an introduction and overview / T.H. Steinberg -- Elution of proteins from gels / R.R. Burgess -- Performing and optimizing Western blots with an emphasis on chemiluminescent detection / A. Alegria-Schaffer, A. Lodge and K. Vattem -- Detergents: an overview / D. Linke -- Purification of membrane proteins / S.H. Lin and G. Guidotti -- Purification of recombinant G-protein-coupled receptors / R. Grisshammer -- Cell-free translation of integral membrane proteins into unilamelar liposomes / M.A. Goren ... [et al.] -- Determination of protein purity / D.G. Rhodes and T.M. Laue -- Determination of size, molecular weight, and presence of subunits / D.G. Rhodes, R.E. Bossio and T.M. Laue -- Identification and quantification of protein posttranslational modifications / A.R. Farley and A.J. Link -- Parallel methods for expression and purification / S.A. Lesley -- Techniques to isolate O2-sensitive proteins: [4Fe-4S]-FNR as an example / A. Yan and P.J. Kiley -- Rethinking your purification procedure / M.P. Deutscher -- Important but little known (or forgotten) artifacts in protein biochemistry / R.R. Burgess.
588			_aDescription based on print version record.
520			_aThe second edition of this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology, biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice in order to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines including biochemistry, genetics, oncology, pharmacology, dermatology and immunology. Assembles chapters on both common and less-common relevant techniques. Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process.
650		0	_aProteins _xPurification.
650	1	2	_aProteins _xisolation & purification.
650		7	_aProteins _xPurification. _2fast _0(OCoLC)fst01079750
655		4	_aElectronic books.
700	1		_aBurgess, Richard R.
700	1		_aDeutscher, Murray P.
776	0	8	_iPrint version: _tGuide to protein purification. _b2nd ed. _dLondon : Academic, 2009 _z9780123745361 _w(OCoLC)423582245
830		0	_aMethods in enzymology ; _vv. 463.
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/book/9780123745361
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/bookseries/00766879/463
938			_aBaker and Taylor _bBTCP _nBK0008324600
938			_aYBP Library Services _bYANK _n3017446
938			_aCoutts Information Services _bCOUT _n15046973
938			_aebrary _bEBRY _nebr10408153
942			_cEB
994			_aC0 _bTEF
999			_c21346 _d21346