000			04297cam a2200637Ia 4500
001			ocn668081554
003			OCoLC
005			20141103172216.0
006			m o d
007			cr mnu||||||||
008			101004s2010 caua ob 101 0 eng d
040			_aUMC _beng _epn _cUMC _dE7B _dEBLCP _dSCB _dOCLCQ _dMKC _dCDX _dOPELS _dN$T _dOCLCQ _dDEBSZ _dOCLCQ _dOPELS _dVVL _dOCLCO _dKUK _dOCLCQ
019			_a671244549 _a694146733 _a712995409
020			_a9780080956954 (electronic bk.)
020			_a0080956955 (electronic bk.)
020			_z9780123749062
020			_z0123749069
029	1		_aAU@ _b000046072676
029	1		_aDEBSZ _b372889107
035			_a(OCoLC)668081554 _z(OCoLC)671244549 _z(OCoLC)694146733 _z(OCoLC)712995409
050		4	_aQH324.9.C7 _bC79 2010eb
060		4	_aQU135 _b.M49 v.481
072		7	_aSCI _x023000 _2bisacsh
082	0	4	_a502.825 _222
049			_aTEFA
245	0	0	_aCryo-EM. _nPart A, _pSample preparation and data collection _h[electronic resource] / _cedited by Grant J. Jensen.
260			_aSan Diego, Calif : _bAcademic Press/Elsevier, _c�2010.
300			_a1 online resource (xlv, 410 pages) : _billustrations.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
490	1		_aMethods in enzymology, _x0076-6879 ; _vv. 481
504			_aIncludes bibliographical references and indexes.
500			_aTitle from PDF title page (ScienceDirect, viewed Oct. 4, 2010).
505	0		_aPart A: Sample preparation and data collection. 3d reconstruction from electron micrographs a personal account of its development -- Preparation of 2d crystals of membrane proteins for high-resolution electron crystallography data collection -- Helical crystallization of soluble and membrane binding proteins -- Plunge freezing for electron cryomicroscopy -- A practical guide to the use of monolayer purification and affinity grids -- Grafix: Stabilization of fragile macromolecular complexes for single particle cryo-EM -- Cryonegative staining of macromolecular assemblies -- Liposomes on a streptavidin crystal: A system to study membrane proteins by cryo-EM -- Micromanipulator-assisted vitreous cryosectioning and sample preparation by high-pressure freezing -- Site-specific biomolecule labeling with gold clusters -- How to operate a cryo-electron microscope -- Collecting electron crystallographic data of two-dimensional protein crystals -- Automated data collection for electron microscopic tomography -- Correlated light and electron cryo-microscopy -- Phase plates for transmission electron microscopy -- Radiation damage in electron cryomicroscopy.
520			_aThis volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, intrumentation, and methods underlying all cryo-EM approaches which allow practictioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. * Includes time-tested core methods and new innovations applicable to any researcher * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide.
650		0	_aElectron microscopy _xTechnique.
650		0	_aProteins _xStructure.
650		0	_aGenomics.
650		2	_aMicroscopy, Electron.
650		2	_aProteins.
650		2	_aGenomics.
650		7	_aSCIENCE _xElectron Microscopes & Microscopy. _2bisacsh
655		4	_aElectronic books.
700	1		_aJensen, Grant J.
776	0	8	_iPrint version: _tSample preparation and data collection. _dLondon : Academic, 2010 _z9780123749062 _w(OCoLC)651072624
830		0	_aMethods in enzymology ; _vv. 481. _x0076-6879
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/book/9780123749062
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/bookseries/00766879/481
938			_aCoutts Information Services _bCOUT _n16426394
938			_aEBL - Ebook Library _bEBLB _nEBL648852
938			_aebrary _bEBRY _nebr10427978
938			_aEBSCOhost _bEBSC _n345097
942			_cEB
994			_aC0 _bTEF
999			_c21257 _d21257