000			05544cam a2200637Ia 4500
001			ocm56578168
003			OCoLC
005			20141103172215.0
006			m o d
007			cr |m|n u|||||
008			040927s1999 cauaf obf 001 0 eng
040			_aAZU _beng _cAZU _dOCLCQ _dCUSER _dCOF _dOPELS _dOCLCQ _dOPELS _dOCLCF _dKUK
019			_a326626500
020			_a0121822044 (electronic bk.)
020			_a9780121822040 (electronic bk.)
035			_a(OCoLC)56578168 _z(OCoLC)326626500
050		4	_aQP601 _b.M49 vol. 303
060		4	_aW1 _bME9615K v. 303 1999
060		4	_aQU 58.5 _bC386 1999
082	0	4	_a572.86 _221
049			_aTEFA
245	0	0	_aCDNA preparation and characterization _h[electronic resource] / _cedited by Sherman M. Weissman.
260			_aSan Diego : _bAcademic Press, _cc1999.
300			_a1 online resource (xxxii, 575 p.) : _bill. (some col.)
490	1		_aMethods in enzymology ; _vv. 303
504			_aIncludes bibliographical references and indexes.
505	0		_acDNA preparation -- Gene identification -- Patterns of mRNA expression -- Functional relationship among cDNA translation products.
588			_aDescription based on print version record.
520			_aGenomic sequences, now emerging at a rapid rate, are greatly expediting certain aspects of molecular biology. However, in more complex organisms, predicting mRNA structure from genomic sequences can often be difficult. Alternative splicing, the use of alternative promoters, and orphan genes without known analogues can call present difficulties in the predictions of the structure of mRNAs or even in gene detection. Both computational and experimental methods remain useful for recognizing genes and transcript templates, even in sequenced DNA. Methods for producing full-length cDNAs are important for determining the structures of the proteins the mRNA encodes, the positions of promoters, and the considerable regulatory information for translation that may be encoded in the 5' untranslated regions of the mRNA. Methods for studying levels of mRNA and their changes in different physiological circumstances are rapidly evolving, and the information from this area will rival the superabundance of information derived from genomic sequences. In particular, cDNAs can be prepared even from single cells, and this approach has already yielded valuable information in several areas. To the extent that reliable and reproducible information, both quantitative and qualitative, can be generated from very small numbers of cells, there are rather remarkable possibilities for complementing functional and genetic analysis of developmental patterns with descriptions of changes in mRNAs. Dense array analysis promises to be particularly valuable for the rapid expression pattern of known genes, while other methods such as gel display approaches offer the opportunity of discovering unidentified genes or for investigating species whose cDNAs or genomes have not been studied intensively. Knowledge of mRNA structure, genomic location, and patterns of expression must be converted into information of the function of the encoded proteins. Each gene can be the subject of years of intensive study. Nevertheless, a number of methods are being developed that use cDNA to predict properties or permit the selective isolation of cDNAs encoding proteins with certain general properties such as selective isolation of cDNAs encoding proteins with certain general properties such as subcellular location. This volume presents an update of a number of approaches relevant to the areas referred to above. The technology in this field is rapidly evolving and these contributions represent a "snapshot in time" of the number of currently available and useful approaches to the problems referred to above. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
650		0	_aDNA _vHandbooks, manuals, etc.
650		0	_aMessenger RNA.
650		0	_aGene mapping.
650	1	2	_aDNA, Complementary _xisolation & purification.
650	2	2	_aBase Sequence.
650	2	2	_aChromosome Mapping _xmethods.
650	2	2	_aDNA _xanalysis.
650	2	2	_aDNA, Complementary _xanalysis.
650	2	2	_aRNA, Messenger _xanalysis.
650		4	_aDNA _xAnalysis.
650		4	_aNucleotide sequence.
650		6	_aADN _xGuides, manuels, etc.
650		6	_aARN messager.
650		6	_aCartes chromosomiques.
650		7	_aDNA. _2fast _0(OCoLC)fst00886555
650		7	_aGene mapping. _2fast _0(OCoLC)fst00939625
650		7	_aMessenger RNA. _2fast _0(OCoLC)fst01017394
655		4	_aElectronic books.
655		7	_aHandbooks, manuals, etc. _2fast _0(OCoLC)fst01423877
700	1		_aWeissman, Sherman M.
776	0	8	_iPrint version: _tCDNA preparation and characterization. _dSan Diego : Academic Press, c1999 _z0121822044 _w(OCoLC)41408954
830		0	_aMethods in enzymology ; _vv. 303.
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/book/9780121822040
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/bookseries/00766879/303
942			_cEB
994			_aC0 _bTEF
999			_c21222 _d21222