000			05020cam a2200637Ma 4500
001			ocn652413809
003			OCoLC
005			20141103172214.0
006			m o d
007			cr gn|||||||||
008			100727s2003 cauaf ob 001 0 eng d
040			_aSFB _beng _cSFB _dNRU _dOCLCQ _dOPELS _dOCLCQ _dOPELS _dOCLCF
016	7		_a101188322
016			_aB0536768
020			_a0123525659 (electronic bk.)
020			_a9780123525659 (electronic bk.)
020			_z9780123525659 (case : alk. paper)
020			_z9780123525666 (pbk.)
020			_z0123525667 (pbk.)
029	1		_aDEBSZ _b405334389
035			_a(OCoLC)652413809
050		4	_aQH585 _b.M47 v.71
060		4	_aW1 _bME9615D v.71 2003
060		4	_aWL 102.5 _bN4938 2003
070			_a442 _b.P92 v. 71
082	0	4	_a611.0188 _222
084			_a42.15 _2bcl
049			_aTEFA
245	0	0	_aNeurons _h[electronic resource] : _bmethods and applications for the cell biologist / _cedited by Peter J. Hollenbeck, James R. Bamburg.
260			_aSan Diego : _bAcademic Press, _cc2003.
300			_a1 online resource (xvi, 441 p., [14] p. of plates) : _bill.
490	1		_aMethods in cell biology ; _vv. 71
504			_aIncludes bibliographical references and index.
505	0		_a1. Comparing the properties of neuronal culture systems: a shopping guide for the cell biologist / Peter J. Hollenbeck and James R. Bamburg -- 2. Growing and working with peripheral neurons / Yan He and Peter W. Baas -- 3. Dissection and culturing of chick ciliary ganglion neurons: a system well suited to synaptic study / Barbara W. Bernstein -- 4. The culture of chick forebrain neurons / Steven R. Heidemann ... [et al.] -- 5. Growing and working with spinal motor neurons / Thomas B. Kuhn -- 6. Avian purkinje neuronal cultures: extrinsic control of morphology by cell type and glutamate / Peter L. Jeffrey ... [et al.] -- 7. Culturing hippocampal and cortical neurons / Peter J. Meberg and Matthew W. Miller -- 8. Working with Xenopus spinal neurons in live cell culture / Timothy M. G�omez ... [et al.] -- 9. Culturing neurons from the snail Helisoma / Christopher S. Cohan, James L. Karnes, and Feng-Quan Zhou -- 10. The tibial-1 pioneer pathway: an in vivo model for neuronal outgrowth and guidance / Jennifer Bonner, Kimberly A. Gerrow, and Timothy P. O'Connor -- 11. Techniques to dissect cellular and subcellular function in the Drosophila nervous system / Heinrich J.G. Matthies and Kendal Broadie -- 12. PC12 cells as a model for studies of regulated secretion in neuronal and endocrine cells / T.F.J. Martin and R.N. Grishanin --13. B35 neuroblastoma cells: an easily transfected, cultured cell model of central nervous system neurons / Carol A. Otey, Malika Boukhelifa, and Patricia Maness -- 14. Live-cell imaging of slow axonal transport in cultured neurons / Anthony Brown -- 15. Making proteins into drugs: assisted delivery of proteins and peptides into living neurons / Gianluca Gallo -- 16. Transfection of primary central and peripheral nervous system neurons by electroporation / Cecilia Y. Martinez and Peter J. Hollenbeck -- 17. Biolistic transfection / Paul C. Bridgman, Michael E. Brown, and Irina Balan -- 18. Expression of transgenes in primary neurons from chick peripheral and central nervous systems by retroviral infection of early embryos / Peter J. Hollenbeck and D.M. Fekete -- 19. Production and use of replication-deficient adenovirus for transgene expression in neurons / L.S. Minamide ... [et al.].
520			_aThis book lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented. * Contains culture methodology for more than a dozen typ.
588			_aDescription based on print version record.
650		0	_aNeurons.
650		0	_aCytology.
650	1	2	_aNeurons _xcytology.
650	2	2	_aCell Culture Techniques _xmethods.
650	2	2	_aCytological Techniques.
650		6	_aNeurones _xCytologie.
650		6	_aCellules _xCulture.
650		6	_aCytologie _xTechnique.
650		7	_aCytology. _2fast _0(OCoLC)fst00886282
650		7	_aNeurons. _2fast _0(OCoLC)fst01036436
655		4	_aElectronic books.
700	1		_aHollenbeck, Peter James.
700	1		_aBamburg, James R.
710	2		_aAmerican Society for Cell Biology.
776	0	8	_iPrint version: _tNeurons : _bmethods and applications for the cell biologist. _dSan Diego : _z9780123525659 _z0123525659 _w(OCoLC)52698581
830		0	_aMethods in cell biology ; _vv. 71.
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/book/9780123525659
942			_cEB
994			_aC0 _bTEF
999			_c21138 _d21138