| 000 | 03733cam a2200637Ia 4500 | ||
|---|---|---|---|
| 001 | ocn172840245 | ||
| 003 | OCoLC | ||
| 005 | 20141103172214.0 | ||
| 006 | m o d | ||
| 007 | cr cnu---unuuu | ||
| 008 | 070917s2000 cauaf ob 001 0 eng d | ||
| 040 |
_aN$T _beng _cN$T _dYDXCP _dBTCTA _dOCLCQ _dMUU _dIDEBK _dE7B _dOCLCQ _dOPELS _dOCLCQ _dOPELS _dOCLCF |
||
| 019 |
_a171132492 _a176887091 _a301815826 _a648267429 |
||
| 020 | _a9780080524955 (electronic bk.) | ||
| 020 | _a0080524958 (electronic bk.) | ||
| 020 | _a0125441649 (electronic bk.) | ||
| 020 | _a9780125441643 (electronic bk.) | ||
| 020 | _z0120645254 (pb) | ||
| 029 | 1 |
_aDEBSZ _b405316348 |
|
| 035 |
_a(OCoLC)172840245 _z(OCoLC)171132492 _z(OCoLC)176887091 _z(OCoLC)301815826 _z(OCoLC)648267429 |
||
| 050 | 4 |
_aQH585 _b.M47eb vol. 62 |
|
| 060 | 4 |
_aW1 _bME9615D v.62 2000 |
|
| 060 | 4 |
_aQX 151 _bT348 2000 |
|
| 070 |
_a442 _bP92 v.62 |
||
| 072 | 7 |
_aSCI _x017000 _2bisacsh |
|
| 082 | 0 | 4 |
_a571.6 _222 |
| 049 | _aTEFA | ||
| 245 | 0 | 0 |
_aTetrahymena thermophila _h[electronic resource] / _cedited by David J. Asai, James D. Forney. |
| 260 |
_aSan Diego : _bAcademic Press, _cc2000. |
||
| 300 |
_a1 online resource (xviii, 580 p., [5] p. of plates) : _bill. (some col.) |
||
| 490 | 0 |
_aMethods in cell biology ; _vv. 62 |
|
| 500 | _a"Prepared under the auspices of the American Society for Cell Biology." | ||
| 504 | _aIncludes bibliographical references and index. | ||
| 588 | _aDescription based on print version record. | ||
| 520 | _aTetrahymena thermophila is emerging as a powerful experimental system for functional genetics. The ciliated protozoan offers numerous advantages, not the least of which is the ability to eliminate any specific gene of interest and then to evaluate the effect of that mutation on the living cell. Past investigations with T. thermophila have yielded several key discoveries, including dynein, catalytic RNA, and telomerase. This volume is a comprehensive resource for using Tetrahymena in the laboratory and is aimed at persons already experienced, as well as newcomers to the organism. It covers both the biological background and essential protocols for investigators rapidly turning to Tetrahymena as the experimental system of choice. Key Features * Contains both theoretical and practical issues in 30 chapters contributed by the world authorities on Tetrahymena * Indispensible for both the novice and the experienced researcher * Overviews the history, cell biology, and genetics of the organism * Describes essential protocols on the growth of cells, genetic techniques, and how to look at the cells with the microscope * Illustrates how the methods can be applied to solve various cell biological problems * Reviews recently developed strategies for altering gene expression. | ||
| 650 | 0 | _aTetrahymena. | |
| 650 | 1 | 2 | _aTetrahymena thermophila. |
| 650 | 2 | 2 | _aCell Culture Techniques. |
| 650 | 6 | _aTetrahymena. | |
| 650 | 7 |
_aSCIENCE _xLife Sciences _xCell Biology. _2bisacsh |
|
| 650 | 1 | 7 |
_aProtozoa. _2gtt |
| 650 | 7 |
_aTetrahymena. _2fast _0(OCoLC)fst01148410 |
|
| 655 | 4 | _aElectronic books. | |
| 700 | 1 | _aAsai, David J. | |
| 700 | 1 |
_aForney, James D., _d1957- |
|
| 710 | 2 | _aAmerican Society for Cell Biology. | |
| 776 | 0 | 8 |
_iPrint version: _tTetrahymena thermophila. _dSan Diego : Academic Press, c2000 _z0125441649 _z9780125441643 _w(OCoLC)42352909 |
| 830 | 0 |
_aMethods in cell biology ; _vv. 62. |
|
| 856 | 4 | 0 |
_3ScienceDirect _uhttp://www.sciencedirect.com/science/book/9780125441643 |
| 938 |
_aYBP Library Services _bYANK _n2614340 |
||
| 938 |
_aBaker and Taylor _bBTCP _nBK0007453706 |
||
| 938 |
_aebrary _bEBRY _nebr10179870 |
||
| 938 |
_aEBSCOhost _bEBSC _n196002 |
||
| 942 | _cEB | ||
| 994 |
_aC0 _bTEF |
||
| 999 |
_c21136 _d21136 |
||