000 | 05852cam a2200661Ii 4500 | ||
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001 | ocn861559596 | ||
003 | OCoLC | ||
005 | 20141103172213.0 | ||
006 | m o d | ||
007 | cr |n||||||||| | ||
008 | 131026s2013 vtu o 000 0 eng d | ||
040 |
_aEBLCP _beng _epn _cEBLCP _dOCLCQ _dCUI _dCUS _dE7B _dCDX _dOCLCQ _dN$T _dOPELS _dCUS _dOCLCF _dOCLCQ |
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020 |
_a9780124104709 _q(electronic bk.) |
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020 |
_a0124104703 _q(electronic bk.) |
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020 | _z9780124081437 | ||
020 | _z0124081436 | ||
029 | 1 |
_aAU@ _b000052223954 |
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029 | 1 |
_aDEBSZ _b405358180 |
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035 | _a(OCoLC)861559596 | ||
050 | 4 | _aQH603.C43 | |
072 | 7 |
_aSCI _x056000 _2bisacsh |
|
082 | 0 | 4 |
_a571.64 _223 |
049 | _aTEFA | ||
245 | 0 | 0 |
_aReceptor-receptor interactions _h[electronic resource] / _cedited by P. Michael Conn. |
260 |
_aBurlington : _bElsevier Science, _c2013. |
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300 | _a1 online resource (539 pages). | ||
336 |
_atext _btxt _2rdacontent |
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337 |
_acomputer _bc _2rdamedia |
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338 |
_aonline resource _bcr _2rdacarrier |
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490 | 1 |
_aMethods in cell biology ; _vv. 117 |
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505 | 0 | _aFront Cover; Receptor-Receptor Interactions; Copyright; Contents; Contributors; Preface; References; Chapter 1: Spatial Intensity Distribution Analysis (SpIDA) : A New Tool for Receptor Tyrosine Kinase Activation and Transactivation Quantification; Introduction; 1.1. Theory of Spatial Intensity Distribution Analysis; 1.1.1. Theoretical basis of SpIDA; 1.2. SpIDA: Examples of Application to RTK; 1.2.1. Quantification of EGFR-eGFP; 1.2.2. Application to RTK transactivation in a native system: neurons expressing dopamine receptors; 1.3. Procedure for SpIDA; 1.3.1. Material and apparatus. | |
505 | 8 | _a1.3.1.1. Sample preparation; 1.3.2. Necessary experimental controls : defining the QB for monomeric moiety; 1.3.2.1. Determining the monomeric QB for receptors tagged with fluorescent proteins; 1.3.2.2. Determining the monomeric QB for antibody labeling using detection of a reference monomeric protein; 1.3.2.3. Determining the monomeric QB by using pharmacological agents that block oligomerization and induce monomeric conformation; 1.3.3. Image acquisitions; 1.3.4. Analysis with the SpIDA program graphical user interface; 1.3.4.1. Description of histogram parameters; 1.3.4.2. SpIDA GUI procedures. | |
505 | 8 | _a1.3.5. Determination of analog detector signal broadening; 1.3.5.1. Analog detector calibration procedure; 1.3.6. Data interpretation and pharmacological analysis; 1.4. Discussion; Acknowledgments; References; Chapter 2: Dimerization of Nuclear Receptors; Introduction; 2.1. Methods; 2.1.1. Studies of NR-NR interactions through protein crystallization; 2.1.1.1. Required materials; 2.1.1.2. Protocol; 2.1.2. Expression and purification of NR-NR complexes; 2.1.2.1. Required materials; 2.1.2.2. Protocol; 2.1.3. Monitoring NR-NR interactions by noncovalent electrospray ionization mass spectrometry. | |
505 | 8 | _a2.1.3.1. Required materials2.1.3.2. Protocol; 2.1.4. Monitoring NR-NR interactions by electrophoretic mobility shift assays; 2.1.4.1. Required materials; 2.1.4.2. Protocol; 2.1.4.2.1. In vitro transcription-translation; 2.1.4.2.2. Electrophoretic mobility shift assay; 2.1.5. Two-hybrid assays to define NR-NR interactions in living cells; 2.1.5.1. Required materials; 2.1.5.2. Protocol; 2.1.5.2.1. Transient transfection of HeLa cells; 2.1.5.2.2. Cell lysis; 2.1.5.2.3. Preparation of luciferase assay; 2.1.5.2.4. Luciferase measurement; 2.1.5.2.5. Measurement of {Beta]gal activity. | |
505 | 8 | _a2.1.5.2.6. Normalization; 2.1.6. Fluorescence cross-correlation spectroscopy to measure the concentrations and interactions of NRs in living cells; 2.1.6.1. Required materials; 2.1.6.2. Protocol; 2.1.6.2.1. Cell culture and transfection; 2.1.6.2.2. Fluorescence cross-correlation microscopy; 2.1.6.2.3. Data analysis; Acknowledgments; References; Chapter 3 : Network Analysis to Uncover the Structural Communication in GPCRs; Introduction; 3.1. Materials; 3.2. Methods; 3.2.1. Workflow of the PSN-MD and PSN-ENM approaches; 3.2.2. Building the PSG; 3.2.3. Search for the shortest communication paths. | |
500 | _a3.3. Discussion. | ||
520 | _aThis new volume of Methods in Cell Biology looks at receptor-receptor interactions, with sections on allosteric and effector interactions, crystallization and modeling, measuring receptor-receptor interactions and oligomerization in individual classes. With cutting-edge material, this comprehensive collection is intended to guide researchers of receptor-receptor interactions for years to come. Covers sections on allosteric and effector interactions, crystallization and modeling, measuring receptor-receptor interactions and oligomerization in individual class. | ||
588 | 0 | _aPrint version record. | |
650 | 0 | _aCell receptors. | |
650 | 2 | _aReceptors, Cell Surface. | |
650 | 2 | _aSignal Transduction. | |
650 | 2 | _aStructure-Activity Relationship. | |
650 | 7 |
_aSCIENCE _xLife Sciences _xAnatomy & Physiology. _2bisacsh |
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650 | 7 |
_aCell receptors. _2fast _0(OCoLC)fst00850227 |
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655 | 4 | _aElectronic books. | |
700 | 1 |
_aConn, P. Michael, _eeditor. |
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776 | 0 | 8 |
_iPrint version: _aConn, P. Michael. _tReceptor-Receptor Interactions : Methods in Cell Biology. _dBurlington : Elsevier Science, �2013 _z9780124081437 |
830 | 0 |
_aMethods in cell biology ; _vv. 117. |
|
856 | 4 | 0 |
_3ScienceDirect _uhttp://www.sciencedirect.com |
856 | 4 | 0 |
_3ScienceDirect _uhttp://www.sciencedirect.com/science/bookseries/0091679X/117 |
856 | 4 | 0 |
_3ScienceDirect _uhttp://www.sciencedirect.com/science/book/9780124081437 |
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