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001 ocn880827118
003 OCoLC
005 20141103172213.0
006 m o d
007 cr cnu---unuuu
008 140531t20142014onc o 000 0 eng d
040 _aEBLCP
_beng
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_dN$T
_dUIU
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020 _a9780124171787 (electronic bk.)
020 _a0124171788 (electronic bk.)
020 _z9780124171602
029 1 _aDEBSZ
_b414275993
035 _a(OCoLC)880827118
050 4 _aQH595
_b.N384 2014eb
072 7 _aSCI
_x008000
_2bisacsh
082 0 4 _a574.87/32
_223
049 _aTEFA
245 0 0 _aNuclear pore complexes and nucleocytoplasmic transport - Methods /
_cedited by Val�erie Doye.
250 _aFirst edition.
264 1 _aSan Diego, CA :
_bAcademic Press,
_c[2014].
264 4 _c�2014
300 _a1 online resource (xx, 508 pages) :
_bcolor illustrations.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
490 1 _aMethods in Cell Biology ;
_vvolume 122
500 _aDescription based upon print version of record.
505 0 _aFront Cover; Nuclear Pore Complexes and Nucleocytoplasmic Transport -- Methods; Copyright; Contents; Contributors; Preface; Chapter 1: Fifty Years of Nuclear Pores and Nucleocytoplasmic Transport Studies: Multiple Tools Revealing Complex Rules; Introduction; 1.1. The NPCs: A Modular Macromolecular Assembly; 1.1.1. Toward a refined view of the NPC structure; 1.1.2. Nucleoporins: The building blocks of NPCs; 1.1.3. Integrating the Nups into the 3D architecture of the NPC; 1.1.3.1. Nup localization; 1.1.3.2. Interactions among Nups; 1.1.3.3. Nup stoichiometry
505 8 _a1.1.3.4. Toward a detailed map of NPCs1.1.4. Nups are composed of a limited set of structural domains; 1.2. Nucleocytoplasmic Trafficking: The Rules of the Road; 1.2.1. Investigating nucleocytoplasmic transport; 1.2.1.1. Historical overview; 1.2.1.2. Expanding the toolbox; 1.2.2. The signals for nucleocytoplasmic exchanges; 1.2.3. A family of protein transport receptors: The karyopherins; 1.2.4. The Ran GTPase: A key to transport directionality; 1.2.5. The case of INM targeting; 1.2.6. Distinct pathways contribute to RNA export
505 8 _a1.2.7. Translocation across the NPCs: A dual function for FG-Nups as barrier and gate1.2.8. Noncanonical transport pathways through the NE; 1.3. The Nuclear Transport Machinery: A Dynamic and Versatile Device; 1.3.1. NPC biogenesis throughout the cell cycle; 1.3.1.1. NPC disassembly; 1.3.1.2. Post-mitotic NPC assembly; 1.3.1.3. De novo NPC assembly; 1.3.2. Multiple functions of the nuclear transport machinery during the cell cycle; 1.3.3. NPCs, nuclear organization, and gene expression; 1.3.4. NPCs and genetic stability; Concluding Remarks; Acknowledgments; References
505 8 _aChapter 2: Imaging Metazoan Nuclear Pore Complexes by Field Emission Scanning Electron MicroscopyIntroduction; Prehistoric landmarks (before EM): From cells to the nuclear envelope; The EM era: From the nuclear envelope to nuclear pore complexes; High resolution SEM: Direct surface imaging of NPCs; 2.1. Rationale; 2.1.1. Purpose; 2.1.2. An overview of sample preparation; 2.1.2.1. Fixation; 2.1.2.2. Dehydration and critical point drying; 2.1.2.3. Sputter coating; 2.2. Materials; 2.2.1. Equipment; 2.2.2. Materials; 2.2.3. Reagents; 2.2.4. Buffers; 2.3. Anchored Nuclei
505 8 _a2.3.1. Coating silicon chips2.3.2. Chromatin decondensation and attachment; 2.3.3. In vitro assembly reaction; 2.4. Mammalian Cell Nuclei; 2.5. Immunogold Labeling; 2.6. Sample Preparation for FESEM; 2.6.1. Fixation, dehydration, and CPD; 2.6.2. Sputter coating and FESEM analysis; Acknowledgments; References; Chapter 3: Imaging Yeast NPCs: From Classical Electron Microscopy to Immuno-SEM; Introduction; 3.1. Conventional TEM; 3.1.1. Materials; 3.1.1.1. Equipment; 3.1.1.2. Spheroplast preparation; 3.1.1.3. Fixation, dehydration, contrasting, and embedding; 3.1.1.4. Sectioning and poststaining
500 _a3.1.2. Procedure
520 _aVolume 122 of Methods in Cell Biology describes modern tools and techniques used to study nuclear pore complexes and nucleocytoplasmic transport in diverse eukaryotic model systems (including mammalian cells, Xenopus, C. elegans, yeast). The volume enables investigators to analyze nuclear pore complex structure, assembly, and dynamics; to evaluate protein and RNA trafficking through the nuclear envelope; and to design in vivo or in vitro assays appropriate to their research needs. Beyond the study of nuclear pores and transport as such, these protocols will also.
650 0 _aCell nuclei.
650 0 _aNuclear membranes.
650 0 _aCytoplasm.
650 0 _aBiological transport.
650 2 _aCell Nucleus.
650 2 _aNuclear Pore.
650 2 _aActive Transport, Cell Nucleus.
650 2 _aCytological Techniques.
650 4 _aCell biology.
650 4 _aCell nuclei.
650 4 _aScience.
650 7 _aSCIENCE / Life Sciences / Biology
_2bisacsh
650 7 _aBiological transport.
_2fast
_0(OCoLC)fst00832345
650 7 _aCell nuclei.
_2fast
_0(OCoLC)fst00850208
650 7 _aCytoplasm.
_2fast
_0(OCoLC)fst00886318
650 7 _aNuclear membranes.
_2fast
_0(OCoLC)fst01040367
655 4 _aElectronic books.
700 1 _aDoye, Val�erie,
_eeditor.
776 0 8 _iPrint version:
_aDoye, Val�erie.
_tNuclear pore complexes and nucleocytoplasmic transport - Methods.
_dBurlington : Elsevier Science, 2014
_z9780124171787
830 0 _aMethods in cell biology ;
_vv. 122.
856 4 0 _3ScienceDirect
_uhttp://www.sciencedirect.com/science/book/9780124171602
856 4 0 _uhttp://www.sciencedirect.com/science/publication?issn=0091679X&volume=122
938 _aEBL - Ebook Library
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_nEBL1694271
938 _aEBSCOhost
_bEBSC
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942 _cEB
994 _aC0
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999 _c21085
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