000			05554cam a2200721Ia 4500
001			ocn171024777
003			OCoLC
005			20141103172213.0
006			m o d
007			cr cnu---unuuu
008			070912s2001 caua ob 001 0 eng d
040			_aN$T _beng _epn _cN$T _dYDXCP _dBTCTA _dOCLCQ _dMUU _dUX0 _dIDEBK _dE7B _dOCLCQ _dMHW _dOCLCQ _dOPELS _dOCLCQ _dOPELS _dOCLCF _dOCLCQ
019			_a171132293 _a178597441 _a259486260 _a301936895 _a648245977 _a823828109 _a823897694 _a824089165 _a824135568
020			_a9780080522531 (electronic bk.)
020			_a008052253X (electronic bk.)
020			_a012544169X (electronic bk.)
020			_a9780125441698 (electronic bk.)
020			_a1281021075
020			_a9781281021076
029	1		_aDEBSZ _b405316232
029	1		_aNZ1 _b12468886
035			_a(OCoLC)171024777 _z(OCoLC)171132293 _z(OCoLC)178597441 _z(OCoLC)259486260 _z(OCoLC)301936895 _z(OCoLC)648245977 _z(OCoLC)823828109 _z(OCoLC)823897694 _z(OCoLC)824089165 _z(OCoLC)824135568
050		4	_aQH585 _b.M38eb vol. 65
060		4	_aW1 _bME9615D v.65 2001
060		4	_aQH 603.M5 _bM678 2001
070			_a442 _bP92 v. 65
072		7	_aSCI _x017000 _2bisacsh
072		7	_aPSF _2bicssc
082	0	4	_a571.65736 _222
049			_aTEFA
245	0	0	_aMitochondria _h[electronic resource] / _cedited by Liza A. Pon, Eric A. Schon.
260			_aSan Diego, Calif. : _bAcademic Press, _c�2001.
300			_a1 online resource (xvii, 519 pages, [11] pages of illustrations) : _billustrations (some color).
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
490	1		_aMethods in cell biology, _x0091-679X ; _vv. 65
500			_a"Prepared under the auspices of the American Society for Cell Biology."
504			_aIncludes bibliographical references and index.
505	0		_aIsolation and subfractionation of mitochondria from animal cells and tissue culture lines -- Isolation and subfractionation of mitochondria from the yeast Saccharomyces cerevisiae -- Isolation and subfractionation of mitochondria from plants -- Assessing functional integrity of mitochondria in vitro and in vivo -- Assaying mitochondrial respiratory complex activity in mitochondria isolated from human cells and tissues -- In vivo measurements of respiration control by cytochrome c oxidase and in situ analysis of oxidative phosphorylation -- Assay of mitochondrial ATP synthesis in animal cells -- Measurement of membrane permeability and permeability transition of mitochondria -- Assaying actin-binding activity of mitochondria in yeast -- Analysis and prediction of mitochondrial targeting peptides -- Assaying protein import into mitochondria -- Analysis of protein-protein interactions in mitochondria by coimmunoprecipitation and chemical cross-linking -- Blue-native gels to isolate protein complexes from mitochondria -- Application of electron tomography to mitochondrial research -- Epitope tagging and visualization of nuclear-encoded mitochondrial proteins in yeast -- Targeting of green fluorescent protein to mitochondria -- Assessment of mitochondrial membrane potential in situ using single potentiometric dyes and a novel fluorescence resonance energy transfer technique -- Optical imaging techniques (histochemical, immunohistochemical, and in situ hybridization staining methods) to visualize mitochondria -- Visualization of mitochondrial movement in yeast -- Targeting of reporter molecules to mitochondria to measure calcium, ATP, and pH -- Genetic transformation of Saccharomyces cerevisiae mitochondria -- Transmitochondrial technology in animal cells -- Diagnostic assays for defects in mitochondrial DNA replication and transcription in yeast and human cells -- Analysis of mitochondrial translation products in Vivo and in organello in yeast -- Numerical methods for handling uncertainty in microarray data: An example analyzing perturbed mitochondrial function in yeast.
520			_aThis title employs biochemical, cell biological, and genetic approaches to study mitochondrial structure, function, and biogenesis. Also of interest are the consequences of impaired mitochondrial function on cells, tissues, and organs. The book is full of step-by-step "how to" methods with sample results, interpretations, and pitfalls. There is a unique set of appendices that include gene catalogs, mtDNA maps, and reagents for probing respiratory chain function. Finally, there are applications of state-of-the art microarray and gene chip technologies.
588			_aDescription based on print version record.
650		0	_aMitochondria.
650	1	2	_aMitochondria.
650		6	_aMitochondries.
650		7	_aSCIENCE _xLife Sciences _xCell Biology. _2bisacsh
650		7	_aMitochondria. _2fast _0(OCoLC)fst01024016
650	1	7	_aMitochondri�en. _2gtt
655		4	_aElectronic books.
700	1		_aPon, Liza A.
700	1		_aSchon, Eric A.
710	2		_aAmerican Society for Cell Biology.
776	0	8	_iPrint version: _tMitochondria. _dSan Diego, Calif. : Academic Press, �2001 _z012544169X _z9780125441698 _w(OCoLC)46393271
830		0	_aMethods in cell biology ; _vv. 65.
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/book/9780125441698
938			_aBaker and Taylor _bBTCP _nBK0007446529
938			_aebrary _bEBRY _nebr10175670
938			_aEBSCOhost _bEBSC _n196592
938			_aYBP Library Services _bYANK _n2614202
938			_aIngram Digital eBook Collection _bIDEB _n102107
942			_cEB
994			_aC0 _bTEF
999			_c21080 _d21080