000 02664cam a2200445La 4500
001 ocn648265494
003 OCoLC
005 20141103172213.0
006 m o u
007 cr cn|||||||||
008 070326s2007 enkaf ob 001 0 eng d
040 _aE7B
_beng
_cE7B
_dOCLCQ
_dOPELS
_dOCLCQ
_dOCLCF
020 _a0123740258 (electronic bk.)
020 _a9780123740250 (electronic bk.)
029 1 _aDEBSZ
_b405332971
035 _a(OCoLC)648265494
050 4 _aQH222
_b.D54 2007eb
082 0 4 _a570.282
_222
049 _aTEFA
245 0 0 _aDigital microscopy
_h[electronic resource] /
_cedited by Greenfield Sluder, David E. Wolf.
250 _a3rd ed.
260 _aOxford :
_bAcademic,
_c2007.
300 _a1 online resource (xvii, 608 p., [18] p. of plates) :
_bill.
490 1 _aMethods in cell biology ;
_vv. 81
504 _aIncludes bibliographical references and index.
588 _aDescription based on print version record.
520 _aThe previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe's theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution. * Expands coverage to include discussion of confocal microscopy not found in the previous edition * Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods.
650 0 _aVideo microscopy.
650 0 _aMicroscopy.
650 7 _aMicroscopy.
_2fast
_0(OCoLC)fst01020062
650 7 _aVideo microscopy.
_2fast
_0(OCoLC)fst01166449
655 4 _aElectronic books.
700 1 _aSluder, Greenfield.
700 1 _aWolf, D. E.
830 0 _aMethods in cell biology ;
_vv. 81.
856 4 0 _3ScienceDirect
_uhttp://www.sciencedirect.com/science/book/9780123740250
938 _aebrary
_bEBRY
_nebr10178501
942 _cEB
994 _aC0
_bTEF
999 _c21050
_d21050