000			06571cam a2200685Ma 4500
001			ocn441779195
003			OCoLC
005			20141103172212.0
006			m o d
007			cr zn|||||||||
008			041201s2004 ne af ob 001 0 eng d
040			_aMERUC _beng _epn _cMERUC _dOCLCQ _dN$T _dIDEBK _dE7B _dOCLCQ _dOPELS _dOCLCQ _dOCLCF _dOCLCQ
016	7		_a101239142 _2DNLM
016	7		_a013121982 _2Uk
019			_a289096556 _a648270670
020			_a0080496601 (electronic bk.)
020			_a9780080496603 (electronic bk.)
020			_a9780080859736 (electronic bk.)
020			_a0080859739 (electronic bk.)
020			_a0125641702 (electronic bk.)
020			_a9780125641708 (electronic bk.)
029	1		_aDEBSZ _b405325495
035			_a(OCoLC)441779195 _z(OCoLC)289096556 _z(OCoLC)648270670
050		4	_aQH581.2 _bC98 2004eb
070			_a442 _bP92 v. 75
072		7	_aSCI _x017000 _2bisacsh
082	0	4	_a571.6028 _222
049			_aTEFA
245	0	0	_aCytometry _h[electronic resource] : _bnew developments / _cedited by Zbigniew Darzynkiewicz, Mario Roederer, Hans Tanke.
250			_a4th ed.
260			_aAmsterdam ; _aLondon : _bElsevier Academic Press, _c�2004.
300			_a1 online resource (xxxvii, 876, [34] pages of plates) : _billustrations (some color).
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
490	1		_aMethods in cell biology, _x0091-679X ; _vv. 75
504			_aIncludes bibliographical references and index.
505	0		_aPart I: Instrumentation/Fluorochromes -- Part II: General Techniques -- Part III: Immunology/T-Cell Responses -- Part III: Immunology/T-Cell Responses -- Part IV: Multi-Color Immunophenotyping -- Part V: Other Topics in Immunology -- Part VI: Cytogenetics.
520			_aThe chapters in CYTOMETRY MCB volumes, including this 4th Edition, provide comprehensive description of particular cytometric methods and review their applications. Some chapters also describe new instrumentation and provide fundamental information on use of new fluorescent probes and on data analysis. Although the term "edition" suggests the update of earlier volumes, in fact, nearly all chapters of the 4th Edition are devoted to new topics. The authors were invited to present not only technical protocols, such as available in other methodology books that specialize in the protocol format, but also to discuss the aspects of the methodology that generally are not included in the protocols. Many chapters, thus, present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical setting, common traps and pitfalls, problems with data interpretation, comparison with alternative assays, choice of the optimal assay, etc. Some chapters review applications of cytometry and complementary methodologies to particular biological problems or clinical tasks. The 34 chapters presented in CYTOMETRY 4th Edition cover a wide range of diverse topics. Several chapters describe progress in technology of fluorescence measurement. The novel phenomenon of the surface-plasmon coupled emission (SPCE) presented in one of these chapters, in combination with nanophotonic technology, is expected to open entirely new possibilities for ultra-sensitive detection of fluorescence, likely from single fluorochrome molecules. The chapter on cytometry of fluorescence resonance energy transfer (FRET) describes the theoretical foundations- and uncovers further analytical possibilities- of this methodology. The chapter on fluorescent proteins is an exhaustive review of accomplishments and possibilities offered in this rapidly expanding field. The critical assessment of quantitative analytical capabilities of confocal microscopy, optimization of emission optics and further progress in development of laser scanning cytometry instrumentation are the topics of other chapters focused on methodology of fluorescence measurement. Another group of chapters describe variety of cytometric methods and their applications in studies of cell death, particularly by mode of apoptosis, mechanism of antitumor drug action, and DNA damage. The rapidly growing application of cytometry in phytoplankton is also assessed in great detail. The chapters on biohazard sorting and data analysis guidelines will be of interest to a variety of researchers in different fields. Cell immunophenotyping represents the most common application of cytometry, particularly in clinical setting. It is not surprising, therefore, that over a third of all chapters of this volume are devoted to methods and applications of multi-color immunophenotyping and probing immune functions of lymphocytes and phagocytes. The field of cytogenetics and molecular genetics is represented by several chapters. Presented are the methods for telomere length measurement and genomic array technology as well as multiplex amplifiable probe hybridization (MAPH) and multiplex ligation -dependent probe amplification (MLPA), the latter to probe the copy number changes (deletions, multiplications) in genomic DNA. A very exhaustive is the chapter describing the use of subtelometric probes in studies of mental retardation. In tradition with the earlier CYTOMETRY editions, the chapters were prepared by the researchers who either developed the described methods, contributed to their modification, or found new applications and have extensive experience in their use. The list of authors, thus, is a continuation of "Who's Who" directory in the field of cytometry.
588			_aDescription based on print version record.
650		0	_aFlow cytometry.
650		0	_aCytophotometry.
650		0	_aCytogenetics.
650		7	_aSCIENCE _xLife Sciences _xCell Biology. _2bisacsh
650		7	_aCytogenetics. _2fast _0(OCoLC)fst00886262
650		7	_aCytophotometry. _2fast _0(OCoLC)fst00886317
650		7	_aFlow cytometry. _2fast _0(OCoLC)fst00927770
655		4	_aElectronic books.
700	1		_aDarzynkiewicz, Zbigniew.
700	1		_aRoederer, Mario.
700	1		_aTanke, H. J.
776	0	8	_iPrint version: _tCytometry. _b4th ed. _dAmsterdam : Elsevier, �2004 _z0124802834 _z9780124802834 _w(OCoLC)253916464
830		0	_aMethods in cell biology ; _vv. 75.
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/book/9780125641708
938			_aebrary _bEBRY _nebr10180581
938			_aEBSCOhost _bEBSC _n241766
938			_aIngram Digital eBook Collection _bIDEB _n101217
942			_cEB
994			_aC0 _bTEF
999			_c21045 _d21045