Single molecule tools. Part B, Super-resolution, particle tracking, multiparameter and force based methods [electronic resource] / edited by Nils G. Walter.
Material type: TextSeries: Methods in enzymology ; v. 475.Publication details: San Diego, CA : Academic Press/Elsevier, 2010. Edition: 1st edDescription: 1 online resource (l, 663 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9780123814838 (electronic bk.); 0123814839 (electronic bk.); 9780123814821 (electronic bk.); 0123814820 (electronic bk.)Other title: Super-resolution, particle tracking, multiparameter and force based methodsSubject(s): Fluorescence spectroscopy | Fluorescent probes | Luminescence spectroscopy | Luminescent probes | Biosensors | Spectrometry, Fluorescence -- Laboratory Manuals | Fluorescence Resonance Energy Transfer -- Laboratory Manuals | Fluorescence -- Laboratory Manuals | Luminescence -- Laboratory Manuals | Biosensing Techniques -- Laboratory Manuals | Molecular Probes -- Laboratory Manuals | Science | Natural history | SCIENCE -- Life Sciences -- Molecular Biology | Biosensors | Fluorescence spectroscopy | Fluorescent probes | Luminescence spectroscopy | Luminescent probesGenre/Form: Electronic books.Additional physical formats: Print version:: Single molecule tools.DDC classification: 572.8 LOC classification: QP601 | .M49eb vol. 475Online resources: ScienceDirect | ScienceDirect Summary: Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. This volume captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. * Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines.Includes bibliographical references and indexes.
Print version record.
Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. This volume captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. * Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines.
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