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The Zebrafish [electronic resource] : biology / edited by H. William Detrich III, Monte Westerfield, Leonard I. Zon.

Contributor(s): Detrich, H. William | Westerfield, Monte | Zon, Leonard I | American Society for Cell BiologyMaterial type: TextTextSeries: Methods in cell biology ; v. 59.Publication details: San Diego : Academic Press, �1999. Description: 1 online resource (xviii, 391, [23] pages of plates) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9780080859576 (electronic bk.); 0080859577 (electronic bk.); 0125441614 (electronic bk.); 9780125441612 (electronic bk.)Subject(s): Zebra danio -- Physiology | Zebra danio -- Genetics | Zebra danio | Zebra danio -- Research -- Methodology | Zebrafish -- physiology | Gene Expression Regulation, Developmental | Zebrafish -- embryology | Zebrafish -- genetics | Danio z�ebr�e -- Physiologie | Danio z�ebr�e -- G�en�etique | Danio z�ebr�e (Animal de laboratoire) | SCIENCE -- Life Sciences -- Zoology -- Ichthyology & Herpetology | NATURE -- Animals -- FishGenre/Form: Electronic books.Additional physical formats: Print version:: Zebrafish.DDC classification: 597.482 LOC classification: QH585 | .M47eb vol. 59NLM classification: W1 | QL 638.C94Online resources: ScienceDirect
Contents:
Overview of the Zebrafish System -- Cell Cycles and Development in the Embryonic Zebrafish -- Zebrafish Embryonal Cell Culture -- Primary Fibroblast Cell Culture -- Production of Haploid and Diploid Androgenetic Zebrafish (Including Methodology for Delayed in Vitro Fertilization) --Analysis of Protein and Gene Expression -- Strategies to Perturb Zebrafish Development -- Vectors and Techniques for Ectopic Gene Expression in Zebrafish -- Analysis of Zebrafish Development Using Explant Culture Assays -- Embryonic Axis Formation in the Zebrafish -- Confocal Microscopic Analysis of Morphogenetic Movements -- Cytoskeletal Dynamics of the Zebrafish Embryo -- Kinesin-like Microtubule Motors in Early Development -- Techniques in Neural Development -- Development of the Retina -- Growth Control in the Ontogenetic and Regenerating Zebrafish Fin -- Vascular and Blood Gene Expression -- Analysis of Hemostasis in the Zebrafish -- Cell Lineage Tracing in Heart Development -- Neurogenesis in Zebrafish Embryos.
Summary: This is the first in a two-volume, comprehensive treatment of the methodologies used in researching the Zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications.
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"Prepared under the auspices of the American Society for Cell Biology."

Includes bibliographical references and index.

Overview of the Zebrafish System -- Cell Cycles and Development in the Embryonic Zebrafish -- Zebrafish Embryonal Cell Culture -- Primary Fibroblast Cell Culture -- Production of Haploid and Diploid Androgenetic Zebrafish (Including Methodology for Delayed in Vitro Fertilization) --Analysis of Protein and Gene Expression -- Strategies to Perturb Zebrafish Development -- Vectors and Techniques for Ectopic Gene Expression in Zebrafish -- Analysis of Zebrafish Development Using Explant Culture Assays -- Embryonic Axis Formation in the Zebrafish -- Confocal Microscopic Analysis of Morphogenetic Movements -- Cytoskeletal Dynamics of the Zebrafish Embryo -- Kinesin-like Microtubule Motors in Early Development -- Techniques in Neural Development -- Development of the Retina -- Growth Control in the Ontogenetic and Regenerating Zebrafish Fin -- Vascular and Blood Gene Expression -- Analysis of Hemostasis in the Zebrafish -- Cell Lineage Tracing in Heart Development -- Neurogenesis in Zebrafish Embryos.

This is the first in a two-volume, comprehensive treatment of the methodologies used in researching the Zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications.

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