Yeast gene analysis [electronic resource] / edited by Ian Stansfield and Michael J.R. Stark.

Contributor(s): Stansfield, Ian [editor.] | Stark, Michael J. R [editor.]Material type: Text

TextSeries: Methods in microbiology ; v. 36.Publication details: Amsterdam ; Boston : Elsevier Academic Press, 2007. Edition: Second editionDescription: 1 online resource (xvi, 700 pages, [12] pages of plates) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9780080521787 (electronic bk.); 0080521789 (electronic bk.); 0123694787 (electronic bk.); 9780123694782 (electronic bk.)Subject(s): Microbiology -- Technique | Yeast -- Genetics | Saccharomyces cerevisiae -- genetics | Genetic Techniques | Yeasts -- genetics | SCIENCE -- Life Sciences -- Evolution | Microbiology -- Technique | Yeast -- Genetics | Hefeartige Pilze | Genanalyse | AufsatzsammlungGenre/Form: Electronic books.Additional physical formats: Print version:: Yeast gene analysis.DDC classification: 576.028 LOC classification: QR65 | .N59 2007ebNLM classification: W1 | QW 180.5.Y3Online resources: ScienceDirect | ScienceDirect

Contents:

Chapter 1. Introduction to Functional Analysis in Yeast -- Chapter 2. Yeast Genetics and Strain Construction -- Chapter 3. Yeast Transformation -- Chapter 4. A Guided Tour to PCR-based Genomic Manipulations of S. cerevisiae (PCR-targeting) -- Chapter 5. Studying Essential Genes: Generating and Using Promoter Fusions and Conditional Alleles -- Chapter 6. Yeast Hybrid Approaches -- Chapter 7. Array-Based Yeast Two-Hybrid Screening for Protein-Protein Interactions -- Chapter 8. Reporter Genes and Their Uses in Studying Yeast Gene Expression -- Chapter 9. Transcript Analysis: A Microarray Approach -- Chapter 10. GFP-based Microscopic Approaches for Whole Chromosome Analysis in Yeasts -- Chapter 11. Immunological Methods -- Chapter 12. Measuring the Proximity of Proteins in Living Cells by Fluorescence Resonance Energy Transfer between CFP and YFP -- Chapter 13. Identification, Characterization, and Phenotypic Analysis of Covalently Linked Cell Wall Proteins -- Chapter 14. Yeast Protein Microarrays -- Chapter 15. Smart Genetic Screens -- Chapter 16. High-Throughput Strain Construction and Systematic Synthetic Lethal Screening in Saccharomyces cerevisiae -- Chapter 17. Chemical Genomic Tools for Understanding Gene Function and Drug Action -- Chapter 18. RNA Gene Analysis -- Chapter 19. Analysis of Gene Function of Mitochondria -- Chapter 20. Yeast Prions and Their Analysis In Vivo -- Chapter 21. Metabolic Control in the Eukaryotic Cell, a Systems Biology Perspective -- Chapter 22. Phylogenetic Footprinting -- Chapter 23. Saccharomyces cerevisiae as a Tool for Human Gene Function Discovery -- Chapter 24. Bioinformatic Prediction of Yeast Gene Function -- Chapter 25. Yeast Genetic Strain and Plasmid Collections -- Chapter 26. Yeast Gene Analysis: The Remaining Challenges.

Summary: Focusing on Saccharomyces cerevisiae, the second edition of Yeast Gene Analysis represents a major reworking of the original edition, with many completely new chapters and major revisions to all previous chapters. Originally published shortly after completion of the yeast genome sequence, the new edition covers many of the major genome-wide strategies that have been developed since then such as microarray analysis of transcription, synthetic gene array studies, protein microarrays and chemical genetic approaches. It represents a valuable resource for any research laboratory using budding yeast.

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Includes bibliographical references and index.

Focusing on Saccharomyces cerevisiae, the second edition of Yeast Gene Analysis represents a major reworking of the original edition, with many completely new chapters and major revisions to all previous chapters. Originally published shortly after completion of the yeast genome sequence, the new edition covers many of the major genome-wide strategies that have been developed since then such as microarray analysis of transcription, synthetic gene array studies, protein microarrays and chemical genetic approaches. It represents a valuable resource for any research laboratory using budding yeast.

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