Yeast gene analysis [electronic resource] / edited by Alistair J.P. Brown, Mick Tuite.
Material type:
TextSeries: Methods in microbiology ; v. 26.Publication details: San Diego ; London : Academic, 1998. Description: 1 online resource (xiii, 502 pages, [1] page of plates) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9780080860558 (electronic bk.); 0080860559 (electronic bk.); 0125215266 (electronic bk.); 9780125215268 (electronic bk.); 1281755079; 9781281755070Subject(s): Yeast -- Genetics | Mycorrhizas -- Research -- Methodology | Microbiology -- Technique | Yeast -- Genetics -- Technique | Saccharomyces cerevisiae -- genetics | Genetic Techniques | Levure -- G�en�etique | SCIENCE -- Life Sciences -- Mycology | Gist | GenanalyseGenre/Form: Electronic books.Additional physical formats: Print version:: Yeast gene analysis.DDC classification: 579.5/63 LOC classification: QR65 | .M45eb vol. 26NLM classification: QW 180.5 .A8 Y4 1998Online resources: ScienceDirect | ScienceDirect Includes bibliographical references and index.
Chapter 1. Introduction to Functional Analysis of the Yeast Genome --Chapter 2. Whole Chromosome Analysis -- Chapter 3. The Bioinformatics of the Yeast Genome -- Chapter 4. Transformation of Yeast by the Lithium Acetate/Single-Stranded Carrier DNA/PEG Method -- Chapter 5. PCR-Based Gene Targeting in Saccharomyces cerevisiae -- Chapter 6. Studying Essential Genes: Generating and Using Promoter Fusions and Conditional Alleles -- Chapter 7. Ty Mutagenesis -- Chapter 8. Transcript Analysis -- Chapter 9. Reporter Genes and their Use in Studying Yeast Gene Expression -- Chapter 10. Transposon Tagging I: A Novel System for Monitoring Protein Production, Function and Localization -- Chapter 11. Transposon Tagging II: Exploration of Gene Function and Regulatory Networks in Yeast with the Mini-Mu Transposon -- Chapter 12. Immunological Approaches to the Study of Protein Localization in Yeast -- Chapter 13. Posttranslational Modifications of Secretory Proteins -- Chapter 14. Studying Posttranslational Modifications in Yeast: Intracellular Proteins -- Chapter 15. Two-Hybrid Analysis of Protein-Protein Interactions in Yeast -- Chapter 16. "Smart" Genetic Screens -- Chapter 17. Metabolic Control Analysis as a Tool in the Elucidation of the Function of Novel Genes -- Chapter 18. Identifying Stress Genes -- Chapter 19. Identifying Genes Encoding Components of the Protein Synthesis Machinery of the Yeast Saccharomyces cerevisiae -- Chapter 20. MAP Kinase-Mediated Signal Transduction Pathways -- Chapter 21. Analysis of the Candida albicans Genome -- Chapter 22. Applications of Yeast in the Discovery of Human Gene Function -- Chapter 23. Yeast Mutant and Plasmid Collections --Chapter 24. Yeast Gene Analysis in the Next Millennium -- Appendix I: The Ribosomal Protein Genes of Saccharomyces cerevisiae -- Appendix II: Yeast Growth -- Appendix III: Useful World Wide Web Addresses for Yeast Researchers -- Appendix IV: Nomenclature for Yeast Genes and Proteins.
This volume coincides with the completion of the yeast genome sequence, and considers the issue of what all the genes identified actually do. It is designed to help both experienced researchers and those new to yeast gene analysis identify the experimental approaches.
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