RNA turnover in eukaryotes [electronic resource] : nucleases, pathways and analysis of mRNA decay / edited by Lynne E. Maquat, Megerditch Kiledjian.

Includes bibliographical references and indexes.

Analysis of mRNA decapping / S.W. Liu ... [et al.] -- A kinetic assay to monitor RNA decapping under single- turnover conditions / B.N. Jones ... [et al.] -- Purification and analysis of the decapping activator Lsm1p-7p-Pat1p complex from yeast / S. Tharun -- Reconstitution of recombinant human LSm complexes for biochemical, biophysical, and cell biological studies / B.L. Zaric and C. Kambach -- Regulated deadenylation in vitro / A.C. Goldstrohm, B.A. Hook and M. Wickens -- Cell-free deadenylation assays with Drosophila embryo extracts / M. Jeske and E. Wahle -- Measuring CPEB-mediated cytoplasmic polyadenylation-deadenylation in Xenopus laevis oocytes and egg extracts / J.H. Kim and J.D. Richter -- The preparation and applications of cytoplasmic extracts from mammalian cells for studying aspects of mRNA decay / K.J. Sokoloski, J. Wilusz and C.J. Wilusz -- In vitro assays of 5' to 3'-exoribonuclease activity / O. Pellegrini ... [et al.] -- Reconstitution of RNA exosomes from human and Saccharomyces cerevisiae cloning, expression, purification, and activity assays / J.C. Greimann and C.D. Lima -- Biochemical studies of the mammalian exosome with intact cells / G. Schilders and G.J. Pruijn -- Determining in vivo activity of the yeast cytoplasmic exosome / D. Schaeffer ... [et al.] -- Approaches for studying PMR1 endonuclease-mediated mRNA decay / Y. Otsuka and D.R. Schoenberg -- Methods to determine mRNA half-life in Saccharomyces cerevisiae / J. Coller -- mRNA Decay analysis in Drosophila melanogaster drug-induced changes in glutathione S-transferase D21 mRNA stability / B. Akgul and C.P. Tu -- Measuring mRNA stability during early Drosophila embryogenesis / J.L. Semotok ... [et al.] -- Messenger RNA half-life measurements in mammalian cells / C.Y. Chen, N. Ezzeddine and A.B. Shyu -- Trypanosomes as a model to investigate mRNA decay pathways / S. Archer ... [et al.] -- Cell type-specific analysis of mRNA synthesis and decay in vivo with uracil phosphoribosyltransferase and 4-thiouracil / M.D. Cleary -- Analysis of cytoplasmic mRNA decay in Saccharomyces cerevisiae / D.O. Passos and R. Parker -- Transcriptome targets of the exosome complex in plants / D. Belostotsky -- Sensitive detection of mRNA decay products by use of reverse-ligation-mediated PCR (RL-PCR) / T. Grange -- Tethering assays to investigate nonsense-mediated mRNA decay activating proteins / N.H. Gehring, M.W. Hentze and A.E. Kulozik -- Assays for determining poly(A) tail length and the polarity of mRNA decay in mammalian cells / E.L. Murray and D.R. Schoenberg -- Analyzing P-bodies in Saccharomyces cerevisiae / T. Nissan and R. Parker -- Real-time and quantitative imaging of mammalian stress granules and processing bodies / N. Kedersha ... [et al.] -- Cell biology of mRNA decay / D. Grunwald, R.H. Singer and K. Czaplinski.

Description based on print version record.

Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements, responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. The control of biological processes, such as cellular growth and differentiation, is dependent on how the genetic material within a cell is expressed. The cellular physiology of mRNA-including mRNA processing, transport, localization, and turnover-is central to the process of gene expression. Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway. Expert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover which are central to the process of gene expression. Offers step-by-step lab instructions including necessary equipment and reagents.