Cilia [electronic resource] : model organisms and intraflagellar transport / edited by Stephen M. King and Gregory J. Pazour.

Contributor(s): King, Stephen M | Pazour, Gregory J. (Gregory Joseph)Material type: Text

TextSeries: Methods in cell biology ; v. 93.Publication details: Burlington, MA : Elsevier Academic Press, 2009. Description: 1 online resource (xvi, 390 pages, [16] pages of plates) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9780123813787 (electronic bk.); 0123813786 (electronic bk.); 9780123813770 (electronic bk.); 0123813778 (electronic bk.)Subject(s): Cilia and ciliary motion | Cilia | Biological Transport | Cell Movement | Cell membranes | Flagella (Microbiology) | Science | Natural history | SCIENCE -- Life Sciences -- Cytology | Cilia and ciliary motionGenre/Form: Electronic books.Additional physical formats: Print version:: Cilia.DDC classification: 571.67 LOC classification: QP310.C5 | C563 2009ebNLM classification: QS 532.5.E7Online resources: ScienceDirect

Contents:

Manipulating ciliary protein-encoding genes in Tetrahymena thermophila / Drashti Dave, Dorota Wloga, Jacek Gaertig.-- Approaches for functional analysis of flagellar proteins in African trypanosomes / Michael Oberholzer, Miquel A. Lopez, Katherine S. Ralston, Kent L. Hill.-- Tools for analyzing intraflagellar transport in trypanosomes / Daria Julkowska, Philippe Bastin.-- Schmidtea mediterranea: a model system for analysis of motile cilia / Panteleimon Rompolas, Ramila S. Patel-King, Stephen M. King.-- Targeted gene silencing by RNA interference in Chlamydomonas / Eun-Jeong Kim, Heriberto Cerutti.-- Analysis of cargo transport by IFT and GFP imaging of IFT in Chlamydomonas / Dennis Diener.-- Genetic and phenotypic analysis of flagellar assembly mutants in Chlamydomonas reinhardtii / Carlo Iomini, Jacob E. Till, Susan K. Dutcher.-- Recording and analyzing IFT in Chlamydomonas flagella / William Dentler, Kristyn VaderWaal, Mary E. Porter.-- Total internal reflection fluorescence (TIRF) microscopy of Chlamydomonas flagella / Benjamin D. Engel, Karl-Ferdinand Lechtreck, Tsuyoshi Sakai, Mitsuo Ikebe, George B. Witman, Wallace F. Marshall.-- Purification of IFT particle proteins and preparation of recombinant proteins for structural and functional analysis / Robert H. Behal, Ewelina Betleja, Douglas G. Cole.-- Studying cilia in Zebrafish / Iain Drummond.-- Analysis if IFT kinesins in developing Zebrafish cone photoreceptor sensory cilia / Christine Insinna, Katherine Luby-Phelps, Brian A. Link, Joseph C. Besharse.-- Analysis of intraflagellar transport in C. elegans sensory cilia / Limin Hao, Seyda Acar, James Evans, Guangshuo Ou, Jonathan M. Scholey.-- Functional genomics of intraflagellar transport-associated proteins in C. Elgans.-- Generating conditional mutants to analyze ciliary functions: the use of cre-lox technology to disrupt cilia in specific organs / Amber K. O'Connor, Robert A. Kesterson, Bradley K. Yoder.-- Imaging intraflagellar transport in mammalian primary cilia / Tatiana Y. Besschetnova, Barnali Roy, Jagesh V. Shah.-- Analysis of hedgehog signaling in mouse intraflagellar transport mutants / Hyuk W. Ko, Aimin Liu, Jonathan T. Eggenschwiler.

Summary: "Cilia are highly conserved organelles that serve motile and/or sensory functions. These organelles power cell movement, generate fluid flow in various organs, act as sensors of the extracellular environment and have been modified for various specialized tasks such as light reception and smell. Defects in these ubiquitous organelles lead to a broad array of human genetic disorders that range from polycystic kidney disease, retinal degeneration, epilepsy and infertility to developmental defects such as situs inversus and polydactyly. This volume is the third in a three-part series on cilia that focuses on the use of model organisms to gain insight into ciliary function and on the process of intraflagellar transport (IFT) that is essential for the assembly and maintenance of ciliary structures."--Publisher's description.

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Includes bibliographical references and index.

"Cilia are highly conserved organelles that serve motile and/or sensory functions. These organelles power cell movement, generate fluid flow in various organs, act as sensors of the extracellular environment and have been modified for various specialized tasks such as light reception and smell. Defects in these ubiquitous organelles lead to a broad array of human genetic disorders that range from polycystic kidney disease, retinal degeneration, epilepsy and infertility to developmental defects such as situs inversus and polydactyly. This volume is the third in a three-part series on cilia that focuses on the use of model organisms to gain insight into ciliary function and on the process of intraflagellar transport (IFT) that is essential for the assembly and maintenance of ciliary structures."--Publisher's description.

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