Primary cilia [electronic resource] / edited by Roger D. Sloboda.

Includes bibliographical references and index.

Section I: Background.-- From central rudimentary to primary: the history of an underappreciated organelle whose time has come. The primary silium / Robert A. Bloodgood.-- Origin of the clium: novel approaches to examine a cetriolar evolution hypothesis / Mark C. Alliegro, Peter Satir.-- Section II: Cell biology/biochemistry.-- Using quantitative PCR to identify Kinesin-3 genes that are upregulated during growth arrest in mouse NIH3T3 cells / Rikke I. Thorsteinsson, Seren T. Christensen, Lotte B. Pedersen.-- Methods for the isolation of sensory and primary cilia -- an overview / Kimberly A.P. Mitchell, Gabor Szabo, Angela de S. Otero.-- Isolation of primary cilia for morphological analysis / Bing Huang, Tatyana Masyuk, Nicholas LaRusso.-- Analyzing primary cilia by multiphoton microscopy / Cornelia E. Farnum, Rebecca M. Williams, Eve Donnelly.-- Primary cilia and the cell cycle / Olga V. Plotnikova, Elena N. Pugacheva, Erica A. Golemis.-- Section III: Function.-- Utilizing of conditional alleles to study the role of the primary cilium in obesity / Robert A. Kerterson, Nicolas F. Berbari, Raymond C. Pasek, Bradley K. Yoder.-- Using nucleofectin of siRNA constructs for knockdown of primary cilia in P19. CL6 cancer stem cell differentiation into cardiomyocytes / Christian A. Clement, Lars A. Larsen, Seren T. Christensen.-- Primary cilium as a hedgehog signal transduction machine / Sarah C. Geotz, Polloneal J.R. Ocbina, Kathryn V. Anderson.-- Detecting the surgace localization and cytoplasmic cleavage of membrane-bound proteins / Hannah C. Chapin, Vanathy Rajendran, Anna Capasso, Michael J. Caplan.-- Assay for in vitro budding of ciliary-targeted rhodopsin transport carriers / Dusanka Deretic, Jana Mazelova.-- Immunoelectron microscopy of vesicle transport to the primary cilium of photoreceptor cells / Tina Sedmak, Elisabeth Sehn, Uwe Wolfrum.-- Polycystic kidney disease, cilia, and planar polarity / Luis F. Menezes, Gregory G. Germino.-- Constructing and deconstructing roles for the primary cilium in tissue architecture and cancer / E. Scott Seeley, Mazence V. Nachury.-- Section IV: Posttranslational modifications.-- Polyglutamylation and the Fleer gene / Narendra H. Pathak, Iain A. Drummond.-- Regulation of cilia assembly, disassembly, and length by protein phosphorylation / Muqing Cao, Guihua Li, Junmin Pan.-- Postranslational protein modifications in cilia and flagella / Roger D. Sloboda.

"In recent years, the role of cilia in the study of health, development and disease has been increasingly defined, and new discoveries have made this an exciting and important field of research. This comprehensive volume presents easy-to-follow protocols and detailed background information for researchers working with cilia and flagella."--Publisher's description.

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