Protein engineering [electronic resource] / edited by Dan E. Robertson, Joseph P. Noel.
Material type: TextSeries: Methods in enzymology ; v. 388.Publication details: Amsterdam ; Boston : Elsevier Academic Press, c2004. Description: 1 online resource (xxxvii, 413 p., [16] p. of plates) : ill. (some col.)ISBN: 9780080497259 (electronic bk.); 008049725X (electronic bk.); 0121827933 (electronic bk.); 9780121827939 (electronic bk.)Subject(s): Protein engineering | Proteins -- Synthesis | Genetic engineering | Proteins -- Structure-activity relationships | Genetic Engineering | Protein Conformation | Proteins -- genetics | SCIENCE -- Life Sciences -- Biochemistry | Genetic engineering | Protein engineering | Proteins -- Structure-activity relationships | Proteins -- SynthesisGenre/Form: Electronic books.Additional physical formats: Print version:: Protein engineering.DDC classification: 572/.7 LOC classification: QP601 | .M49 vol. 388TP248.65.P76NLM classification: QU 55Online resources: ScienceDirect | ScienceDirect | VolltextIncludes bibliographical references and indexes.
Section I. Methodology -- Gene site saturation mutagenesis : a comprehensive mutagenesis approach -- Single-stranded DNA family shuffling -- Module shuffling -- SCHEMA-guided protein recombination -- Staggered extension process in vitro DNA recombination -- Using incremental truncation to create libraries of hybrid enzymes -- Random multirecombinant polymerase chain reaction -- Gene library synthesis by structure-based combinatorial protein engineering -- New enzymes from combinatorial library modules -- Construction of protein fragment complementation libraries using incremental truncation -- Section II. Applications: optimization and screening -- GigaMatrix: a novel ultrahigh throughput protein optimization and discovery platform -- High throughput microplate screens for directed protein evolution -- Periplasmic expression as a basis for whole cell kinetic screening of unnatural enzyme reactivities -- Engineering the thermotolerance and pH optimum of family 11 xylanases by site-directed mutagenesis -- Screening for oxidative resistance -- Consensus-based engineering of protein stability: from intrabodies to thermostable enzymes -- Improving the functional expression of N-Carbamoylase by directed evolution using the green fluorescent protein fusion reporter system -- Section III. Applications: directed evolution of enzymatic function -- Directed evolution of lipases and esterases -- Protein engineering of the cytochrome P450 monooxygenase from bacillus megaterium -- Directed evolution of aldolases -- Changing the enantioselectivity of enzymes by directed evolution -- Control of stereoselectivity in phosphotriesterase -- Section IV. Applications: evolution of biosynthetic pathways -- Manipulation and analysis of polyketide synthases -- Reactions catalyzed by mature and recombinant nonribosomal peptide synthetases -- Engineering carotenoid biosynthetic pathways -- Section V. Devices, antibodies, and vaccines -- Directed evolution of bacteriorhodopsin for device applications -- Engineering antibody affinity by yeast surface display -- A conditionally replicating virus as a novel approach toward an HIV vaccine.
Description based on print version record.
This MIE volume covers methods for a multitude of topics among which are computational methods, laboratory methods, enzyme optimization, binding proteins/antibodies, and screening technologies.
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