TY - BOOK AU - Walter,Nils G. TI - Single molecule tools T2 - Methods in enzymology, SN - 9780123814838 (electronic bk.) AV - QP601 .M49eb vol. 475 U1 - 572.8 22 PY - 2010/// CY - San Diego, CA PB - Academic Press/Elsevier KW - Fluorescence spectroscopy KW - Fluorescent probes KW - Luminescence spectroscopy KW - Luminescent probes KW - Biosensors KW - Spectrometry, Fluorescence KW - Laboratory Manuals KW - Fluorescence Resonance Energy Transfer KW - Fluorescence KW - Luminescence KW - Biosensing Techniques KW - Molecular Probes KW - Science KW - Natural history KW - SCIENCE KW - Life Sciences KW - Molecular Biology KW - bisacsh KW - fast KW - Electronic books N1 - Includes bibliographical references and indexes N2 - Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. This volume captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. * Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines UR - http://www.sciencedirect.com/science/book/9780123814821 UR - http://www.sciencedirect.com/science/bookseries/00766879/475 ER -