TY  - BOOK
AU  - Rossi,John J.
AU  - Hannon,Gregory J.
TI  - MicroRNA methods
T2  - Methods in enzymology,
SN  - 9780080551395 (electronic bk.)
AV  - QP601 .C733 2007eb
U1  - 572.88 22
PY  - 2007///
CY  - San Diego, Calif.
PB  - Academic Press
KW  - Small interfering RNA
KW  - SCIENCE
KW  - Life Sciences
KW  - Molecular Biology
KW  - bisacsh
KW  - fast
KW  - MicroRNAs
KW  - RNA, Small Interfering
KW  - Electronic books
N1  - Includes bibliographical references and indexes; Ch. 1; Identification of viral microRNAs; Christopher S. Sullivan and Adam Grundhoff --; Ch. 2; Robust machine learning algorithms predict microRNA genes and targets; Pal Saetrom and Ola Snove, Jr. --; Ch. 3; Idenfication of virally encoded microRNAs; Sebastien Pfeffer --; Ch. 4; Computational methods for microRNA target prediction; Yuka Watanabe, Masaru Tomita, and Akio Kanai --; Ch. 5; In vitro and in vivo assays for the activity of Drosha complex; Yoontae Lee and V. Narry Kim --; Ch. 6; Microarray analysis of miRNA gene expression; J. Michael Thomson, Joel S. Parker, and Scott M. Hammond --; Ch. 7; Cloning and detecting signature microRNAs from mammalian cells; Guihua Sun, Haitang Lik and Gohn J. Rossi; Ch. 8; Approaches for studying microRNA and small interfering RNA methylation in vitro and in vivo; Zhiyong Yang ... [et al.] --; Ch. 9; Analysis of small RNA profiles during development; Toshiaki Watanabe ... [et al.] --; Ch. 10; Dissecting microRNA-mediated gene regulation and function in T cell development; Tin Ky Mao and Chang-Zheng Chen --; Ch. 11; Investigation of microRNAs alterations in leukemias and lymphomas; George Adrian Calin and Carlo Maria Croce --; Ch. 12; Discovery of pathogen-regulated small RNAs in plants; Surekha Katiyar-Agarwal and Hailing Jin --; Ch. 13; Protocols for expression and functional analysis of viral microRNAs; Eva Gottwein and Bryan R. Cullen
N2  - MicroRNAs (miRNA) are tiny bits of genetic material that were unknown nearly 10 years ago but now represent an exciting field of study in biology. Upon their discovery, researchers revealed for the first time a new mechanism by which microRNA can stop the function of messenger RNA (mRNA) by literally cutting it in half, interfering with the normal function of specific messenger RNAs in gene expression. This "expression" of genes that code for essential proteins is essentially what controls whether a cell turns into a liver, lung, or brain cell, for example. Understanding what activates this process - or stops it - is a key to understanding the biological process and builds a foundation for advances in medicine and other fields. This volume in Methods in Enzymology presents valuable methods for studying MicroRNA, with three sections covering identification of MicroRNAs and their targets; Micro RNA expression, maturation and functional analysis; and MicroRNAs and disease
UR  - http://www.sciencedirect.com/science/book/9780123739179
UR  - http://www.sciencedirect.com/science/bookseries/00766879/427
ER  -