TY - BOOK AU - Lorsch,Jon TI - Laboratory methods in enzymology: RNA T2 - Methods in enzymology, SN - 9780124200517 (electronic bk.) AV - QP601 .L33 2013eb U1 - 572/.7 23 PY - 2013/// CY - Amsterdam, Boston PB - Elsevier/Academic Press KW - Enzymes KW - Biochemistry KW - Technique KW - Biotechnology KW - Methodology KW - Molecular biology KW - Sequence Analysis, RNA KW - methods KW - istrumentation KW - SCIENCE / Life Sciences / Biochemistry KW - bisacsh KW - fast KW - Electronic books N1 - Includes bibliographical references and indexes; Computational prediction of RNA secondary structure; Walter N. Moss --; Reverse-transcription PCR (RT-PCR); Julia Bachman --; Northern blotting; Shan L. He and Rachel Green --; Explanatory chapter: nuclease protection assays; Elizabeth Eyler --; In vitro transcription from plasmid or PCR-amplified DNA; Julie L. Brunelle and Rachel Green --; Measurement of in vivo RNA synthesis rates; David S. McPheeters and Jo Ann Wise --; Method for measuring mRNA decay rate in Saccharomyces cerevisiae; Wenqian Hu and Jeff Coller --; Analysis of polysomes from bacteria; Daoming Qin and Kurt Fredrick --; Polysome profile analysis : yeast; Martin Posp�isek and Leos Val�asek --; Polysome analysis of mammalian cells; Shan L. He and Rachel Green --; Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae; Wenqian Hu and Jeff Coller --; Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates; Shimyn Slomovic and Gadi Schuster --; Circularized RT-PCR (cRT-PCR) : analysis of the 5' ends, 3' ends, and poly(A) tails of RNA; Shimyn Slomovic and Gadi Schuster --; RNA radiolabeling; Rishi Porecha and Daniel Herschlag --; Fluorescently labeling synthetic RNAs; Max Greenfield and Daniel Herschlag --; Analysis of RNA by analytical polyacrylamide gel electrophoresis; Alexey Petrov, Albet Tsa, and Joseph D. Puglisi --; RNA purification by preparative polyacrylamide gel electrophoresis; Alexey Petrov, Tinghe Wu, Elisabetta Viani Puglisi, and Joseph D. Puglisi --; Explanatory chapter : nucleic acid concentration determination; Sarah Kolitz and Jon Lorsch --; RNA purification : precipitation methods; Sarah E. Walker and Jon Lorsch --; Reverse transcriptase dideoxy sequencing of RNA; Sarah E. Walker and Jon Lorsch --; RNA structure experimental analysis : chemical modification; Zhili Xu and Gloria Culver --; Structural analysis of RNA backbone using in-line probing; Ali Nahvi and Rachel Green UR - http://www.sciencedirect.com/science/book/9780124200371 UR - http://www.sciencedirect.com/science/bookseries/00766879/530 ER -