TY - BOOK AU - Lorsch,Jon TI - Laboratory methods in enzymology T2 - Methods in enzymology, AV - QP551 U1 - 572/.6 23 PY - 2014/// CY - San Diego, CA PB - Academic Press KW - Proteins KW - Analysis KW - analysis KW - fast KW - Electronic books N1 - Includes bibliographical references and indexes N2 - The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 530 volumes and 40,000 chapters in the collection, this is an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few. This volume brings together a number of core protocols concentrating on protein, carefully written and edited by experts, including: Pulse-chase analysis to measure protein degradation; Labeling a protein with fluorophores using NHS ester derivitization; Immunoaffinity purification of proteins; Proteolytic affinity tag cleavage; Purification of GST-tagged proteins. Indispensable tool for the researcher. Contains step-by-step protocols. This volume focuses on core protocols involving protein UR - http://www.sciencedirect.com/science/bookseries/00766879/536 UR - http://www.sciencedirect.com/science/book/9780124200708 ER -