TY - BOOK AU - Brand,Ludwig AU - Johnson,Michael L. TI - Fluorescence spectroscopy T2 - Methods in enzymology, SN - 9780123745866 (electronic bk.) AV - QP601 .M49450 2008eb U1 - 543.56 22 PY - 2008/// CY - San Diego, Calif., London PB - Academic Press/Elsevier KW - Fluorescence spectroscopy KW - fast KW - Spectrometry, Fluorescence KW - methods KW - Electronic books N1 - Includes bibliographical references and indexes; A method in enzymology for measuring hydrolytic activities in live cell environments / Beverly Z. Packard and Akira Komoriya -- Heterogeneity of fluorescence determined by the method of area-normalized time-resolved emission spectroscopy / N. Periasamy -- Multiparametric probing of microenvironment with solvatochromic fluorescent dyes / Andrey S. Klymchenko and Alexander P. Demchenko -- Site-selective red-edge effects / Alexander P. Demchenko -- Fluorescence approaches to quantifying biomolecular interactions / Catherine A. Royer and Suzanne F. Scarlata -- Forster resonance energy transfer measurements of transmembrane helix dimerization energetics / Mikhail Merzlyakov and Kalina Hristova -- Application of single-molecule spectroscopy in studying enzyme kinetics and mechanism / Jue Shi ... [et al.] -- Ultrafast fluorescence spectroscopy via upconversion : applications to biophysics / Jianhua Xu and Jay R. Knutson -- Use of fluorescence resonance energy transfer (FRET) in studying protein-induced DNA bending / Anatoly I. Dragan and Peter L. Privalov --Fluorescent pteridine probes for nucleic acid analysis / Mary E. Hawkins -- Single-molecule fluorescence methods for the analysis of RNA folding and ribonucleoprotein assembly / Goran Pljevalj�ci�c and David P. Millar -- Using fluorophore-labeled oligonucleotides to measure affinities of protein-DNA interactions / Brian J. Anderson ... [et al.] -- Identifying small pulsatile signals within noisy data : a fluorescence application / Michael L. Johnson ... [et al.] -- Determination of zinc using carbonic anhydrase-based fluorescence biosensors / Rebecca Bozym ... [et al.] -- Instrumentation for fluorescence-based fiber optic biosensors / Richard B. Thompson ... [et al.] N2 - Fluorescence spectroscopy is a type of electromagnetic spectroscopy, using a beam of light, which analyzes fluorescence from a sample. Given its extremely high sensitivity and selectivity, it is an important investigational tool in many areas including material sciences, analytical sciences, and across a broad range of chemical, biochemical and medical research. It has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems. The pharmaceutical industry uses it heavily and it has become a dominating technique in biochemistry and molecular genetics. * Keeps MIE buyers and online subscribers up-to-date with the latest research with this highly used technique. * Provides tried and tested techniques which eliminate searching through many different sources UR - http://www.sciencedirect.com/science/book/9780123745866 UR - http://www.sciencedirect.com/science/bookseries/00766879/450 ER -