TY  - BOOK
AU  - Holt,Jo M.
AU  - Johnson,Michael L.
AU  - Ackers,Gary K.
TI  - Energetics of biological macromolecules
T2  - Methods in enzymology,
SN  - 9780080497174 (electronic bk.)
AV  - QP601 .M49eb vol. 379
U1  - 572/.7 22
PY  - 2004///
CY  - Amsterdam, Boston
PB  - Elsevier/Academic Press
KW  - Macromolecules
KW  - Bioenergetics
KW  - Cooperative binding (Biochemistry)
KW  - Allosteric proteins
KW  - Thermodynamics
KW  - Energy Metabolism
KW  - Macromolecular Systems
KW  - SCIENCE
KW  - Life Sciences
KW  - Biochemistry
KW  - bisacsh
KW  - fast
KW  - Electronic books
N1  - Includes bibliographical references and indexes; 1. Analyzing intermediate state cooperativity in hemoglobin -- 2. Nuclear magnetic resonance spectroscopy in the study of hemoglobin cooperativity -- 3. Evaluating cooperativity in dimeric hemoglobins -- 4. Measuring assembly and binding in human embryonic hemoglobins -- 5. Small-angle scattering techniques for analyzing conformational transitions in hemocyanins -- 6. Multivalent protein-carbohydrate interactions: isothermal titration microcalorimetry studies -- 7. Calorimetric analysis of mutagenic effects on protein-ligand interactions -- 8. Multiple binding of ligands to a linear biopolymer -- 9. Probing site-specific energetics in proteins and nucleic acids by hydrogen exchange and nuclear magnetic resonance spectroscopy -- 10. Fluorescence quenching methods to study protein-nucleic acid interactions -- 11. Thermodynamics, protein modification, and molecular dynamics in characterizing lactose repressor protein: strategies for complex analyses of protein structure-function -- 12. Linked equilibria in biotin repressor function: thermodynamic, structural and kinetic analysis -- 13. Distance parameters derived from time-resolved Forster resonance energy transfer measurements and their use in structural interpretations of thermodynamic quantities associated with protein-DNA interactions
N2  - This volume focuses on the cooperative binding aspects of energetics in biological macromolecules. Methodologies such as NMR, small-angle scattering techniques for analysis, calorimetric analysis, fluorescence quenching, and time resolved FRET measurements are discussed
UR  - http://www.sciencedirect.com/science/book/9780121827830
UR  - http://www.sciencedirect.com/science/bookseries/00766879/379
ER  -