TY  - BOOK
AU  - Epstein,Henry F.
AU  - Shakes,Diane C.
ED  - American Society for Cell Biology.
TI  - Caenorhabditis elegans: modern biological analysis of an organism 
T2  - Methods in cell biology,
SN  - 9780080859460 (electronic bk.)
AV  - QH585 .M47eb vol. 48
U1  - 595.182 22
PY  - 1995///
CY  - San Diego
PB  - Academic Press
KW  - Caenorhabditis elegans
KW  - genetics
KW  - Genetic Techniques
KW  - C�norhabditis elegans
KW  - SCIENCE
KW  - Life Sciences
KW  - Zoology
KW  - General
KW  - bisacsh
KW  - gtt
KW  - Celbiologie
KW  - Genanalyse
KW  - swd
KW  - Molekularbiologie
KW  - Cytologie
KW  - Methode
KW  - Genetik
KW  - Neurobiologie
KW  - Genom
KW  - fast
KW  - Nematodes
KW  - Electronic books
N1  - "Prepared under the auspices of the American Society for Cell Biology."; Includes bibliographical references and index; pt. 1. Genetic and culture methods. Basic culture methods / James A. Lewis, John T. Fleming -- Mutagenesis / Philip Anderson -- Reverse genetics : from gene sequence to mutant worm / Ronald H.A. Plasterk -- Genetic mapping with polymorphic sequence-tagged sites / Benjamin D. Williams -- Genetic dissection of developmental pathways / Linda S. Huang, Paul W. Sternberg -- Mosaic analysis / Robert K. Herman -- Genetic balancers / Mark L. Edgley ... [et al.] -- pt 2. Neurobiology. Genetic pharmacology : interactions between drugs and gene products in Caenorhabditis elegans / James B. Rand, Carl D. Johnson -- Methods of studying behavioral plasticity in Caenorhabditis elegans / Timothy N. Gannon, Catharine H. Rankin -- Laser killing of cells in Caenorhabditis elegans / Cornelia I. Bargmann, Leon Avery -- Electrophysiological methods / Leon Avery, David Raizen, Shawn Lockery -- pt. 3. Cell biology and molecular biology. Cell biology of nematode sperm / Steven W. L'Hernault, Thomas M. Roberts -- Blastomere culture and analysis / Lois G. Edgar -- Whole-mount in situ hybridization for the detection of RNA in Caenorhabditis elegans embryos / Geraldine Seydoux, Andrew Fire -- Fluorescence in situ hybridization for the detection of DNA and RNA / Donna G. Albertson, Rita M. Fishpool, Philip S. Birchall -- Immunofluorescence microscopy / David M. Miller, Diane C. Shakes -- Electron microscopy and three-dimensional image reconstruction / David H. Hall; Proteins and protein assemblies / Henry F. Epstein, Feizhou Liu -- DNA transformation / Craig Mello, Andrew Fire -- Transcription and translation ; Techniques for analyzing transcription and translation / Michael Krause -- pt. 4. Genomics and informatics. The physical map of the Caenorhabditis elegans genome / Alan Coulson ... [et al.] -- Genomic DNA sequencing methods / Anthony Favello, LaDeana Hillier, Richard K. Wilson -- Large-scale complementary DNA sequencing methods / Lucinda L. Fulton, LaDeana Hillier, Richard K. Wilson -- ACeDB and Macace / Frank H. Eeckman, Richard Durbin -- The Worm Community System, release 2.0 (WCSr2) / Laura M. Shoman ... [et al.] -- Appendix A. Additional information and resources -- Appendix B. Caenorhabditis Genetics Center, laboratory designations -- Appendix C. Gene names and descriptions
N2  - The first of its kind, this laboratory handbook emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. Four primary sections--Genetic and Culture Methods, Neurobiology, Cell and Molecular Biology, and Genomics and Informatics--reflect the cross-disciplinary nature of C. elegans research. Because C. elegans is a simple and malleable organism with a small genome and few cell types, it provides an elegant demonstr
UR  - http://www.sciencedirect.com/science/book/9780125641494
ER  -