Thiol redox transitions in cell signaling. Part A, Chemistry and biochemistry of low molecular weight and protein thiols [electronic resource] / edited by Enrique Cadenas, Lester Packer.

Includes bibliographical references and indexes.

Changing paradigms in thiology: from antioxidant defense toward redox regulation / Leopold Floh�e -- Mass spectrometry-based methods for the determination of sulfur and related metabolite concentrations in cell extracts / Emmanuel Godat ... [et al.] -- Use of dimedone-based chemical probes for sulfenic acid detection: evaluation of conditions affecting probe incorporation into redox-sensitive proteins / Chananat Klomsiri ... [et al.] -- Use of dimedone-based chemical probes for sulfenic acid detection: methods to visualize and identify labeled proteins / Kimberly J. Nelson ... [et al.] -- Formation and reactions of sulfenic acid in human serum albumin / Beatriz Alvarez ... [et al.] -- Determination of GSH, GSSG, and GSNO using HPLC with electrochemical detection / Li-Peng Yap ... [et al.] -- Measurement of mixed disulfides including glutathionylated proteins / Raffaella Priora ... [et al.] -- Detection and quantification of protein disulfides in biological tissues: a fluorescence-based proteomic approach / Viviana I. P�erez ... [et al.] -- Measurement and identification of S-glutathiolated proteins / Bradford G. Hill ... [et al.] -- Proteome screens for Cys residues oxidation: the redoxome / Giovanni Chiappetta ... [et al.] -- Identification by MS/MS of disulfides produced by a functional redox transition / Pierluigi Mauri ... [et al.] -- Mass spectrometry approaches for the redox characterization of protein cysteine residues: the case of the transcription factor Pax-8 / Andrea Scaloni and Gianluca Tell -- A simple method to systematically study oxidatively modified proteins in biological samples and its applications / Byoung-Joon Song, Soo-Kyung Suh and Kwan-Hoon Moon -- Direct and indirect detection methods for the analysis of S-nitrosylated peptides and proteins / Federico Torta, Lisa Elviri and Angela Bachi -- A rapid approach for the detection, quantification, and discovery of novel sulfenic acid or S-nitrosothiol modified proteins using a biotin-switch method / Joseph R. Burgoyne and Philip Eaton -- Protein addicts of aldehydic lipid peroxidation products: identification and characterization of protein adducts using an aldehyde/keto-reactive probe in combination with mass spectrometry / Claudia S. Maier ... [et al.].

This volume, along with its companion (volume 474), presents methods and protocols dealing with thiol oxidation-reduction reactions and their implications as they relate to�cell signaling. This first�installment of Cadenas and Packer's two-volume treatment specifically deals with glutathionylation and dethiolation, and peroxide removal by peroxiredoxins/thioredoxins and glutathione peroxidases. The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has b.

Description based on print version record.