Stem cell tools and other experimental protocols [electronic resource] / edited by Irina Klimanskaya, Robert Lanza.

Includes bibliographical references and indexes.

In vitro experimentation and research tools: Human embryo culture / A. Mercader [and others] -- Characterization and evaluation of human embryonic stem cells / C. Xu [and others] -- Feeder-free culture of human embryonic stem cells / M. Amit [and others] -- Transgene expression and RNA interference in embryonic stem cells / H. Zaehres [and others] -- Lentiviral vector-mediated gene delivery into human embryonic stem cells / M. Gropp [and others] -- The use of retroviral vectors for gene transfer into hematopoietic stem cells / J. Holst [and others] -- Engineering embryonic stem cells with recombinase systems / F. Schnutgen [and others] -- Gene trapping in embryonic stem cells / W.L. Stanford [and others] -- Genechips in stem cell research / J. Hipp [and others] -- Microarray analysis of stem cells and differentiation / H.Y. Chang [and others] -- Purification of hematopoietic stem cells using the side population / K.K. Lin [and others] -- Cellular reprogramming / S. Agarwal [and others] -- Tissue engineering and regenerative medicine: Tissue engineering using adult stem cells / D. Eberli [and others] -- Tissue engineering using human embryonic stem cells / S. Cohen [and others] -- Engineering cardiac tissue from embryonic stem cells / X. Guo [and others] -- Mesenchymal stem cells and tissue engineering / N.W. Marion [and others] -- Bone reconstruction with bone marrow stromal cells / W. Liu [and others] -- Engineering three-dimensional tissue structures using stem cells / J. Zoldan [and others] -- Immunogenicity of embryonic stem cells and their progeny / M. Drukker [and others] -- Manufacturing considerations for clinical uses of therapies derived from stem cells / D.J. Weber.

This is the third of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a "cookbook" and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using "conven.

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