RNA processing. Part A, General methods [electronic resource] / edited by James E. Dahlberg, John N. Abelson.

Includes bibliographical references.

Isolation of RNA / Shelby L. Berger ... [et al.] -- Purification of RNA molecules gel techniques / Toshimichi Ikemura, James E. Dahlberg and John N. Abelson -- Purifying RNA by column chromatography / N. Kyle Tanner, James E. Dahlberg and John N. Abelson -- Synthesis of long, capped transcripts in Vitro by SP6 and T7 RNA polymerases / Joel K. Yisraeli ... [et al.] -- Synthesis of small RNAs using T7 RNA polymerase / John F. Milligan ... [et al.] -- In vitro synthesis of end-mature, intron-containing transfer RNAs / Vicente M. Reyes ... [et al.] -- Preparation of precursors to mRNA from mammalian cell nuclei / Selina Chen-Kiang ... [et al.] -- Pulse labeling of heterogeneous nuclear RNA in isolated nuclei / Selina Chen-Kiang ... [et al.] -- Isolation and characterization of yeast ribosomal RNA precursors and preribosomes / Jacobus Klootwuk ... [et al.] -- Preparation of yeast transfer RNA precursors in Vivo / Gayle Knapp, James E. Dahlberg and John N. Abelson -- Dideoxy sequencing of RNA using reverse transcriptase / Chang Shahn ... [et al.] -- RNA fingerprinting / Andrea D. Branch ... [et al.] -- Enzymatic RNA sequencing / Y. Kuchino ... [et al.] -- Characterization of cap structures / Yasuhiro Furuichi ... [et al.] -- Isolation and characterization of branched oligonucleotides from RNA / J. David Reilly ... [et al.] -- Enzymatic approaches to probing of RNA secondary and tertiary structure / Gayle Knapp, James E. Dahlberg and John N. Abelson.

A guide for probing native small nuclear RNA and ribonucleoprotein structures / Alain Krol ... [et al.] -- Phylogenetic comparative analysis of RNA secondary structure / Bryan D. James ... [et al.] -- Mapping the genetic organization of RNA by electron microscopy / Louise T. Chow ... [et al.] -- Computer prediction of RNA structure / Michael Zuker, James E. Dahlberg and John N. Abelson -- RNA pseudoknot: Structure, detection, and prediction / C.W.A. Pleij ... [et al.] -- absorbance melting curves of RNA / Joseph D. Puglisi ... [et al.] -- Nuclease digestion: A method for mapping introns / Shelby L. Berger, James E. Dahlberg and John N. Abelson -- Characterization of RNA molecules by S1 nuclease analysis / Arnold J. Berk, James E. Dahlberg and John N. Abelson -- Primer extension analysis of RNA / William R. Boorstein ... [et al.] -- Modification interference analysis of reactions using RNA substrates / Laura Conway ... [et al.] -- Photoaffinity cross-linking methods for studying RNA-protein interactions / Michelle M. Hanna, James E. Dahlberg and John N. Abelson -- Ultraviolet-induced cross-linking of rna to proteins in vivo / Seraf�in Pinol-Roma ... [et al.] -- Analysis of ultraviolet-induced RNA-RNA cross-links: A means for probing RNA structure-function relationships / Andrea D. Branch ... [et al.] -- analysis of splicing complexes and small nuclear ribonucleoprotein particles by native gel electrophoresis / Maria M. Konarska, James E. Dahlberg and John N. Abelson -- Determination of RNA-protein and RNA-ribonucleoprotein interactions by nuclease probing / Katherine A. Parker ... [et al.] -- Immunoprecipitation of ribonucleoproteins using autoantibodies / Joan A. Steitz, James E. Dahlberg and John N. Abelson -- Electron microscopy of ribonucleoprotein complexes on nascent RNA using miller chromatin spreading method / Yvonne N. Osheim ... [et al.] -- Genetic Methods for identification and characterization of RNA-RNA and RNA-protein interactions / Roy Parker, James E. Dahlberg and John N. Abelson -- Compilation of small nuclear RNA sequences / Ram Reddy, James E. Dahlberg and John N. Abelson -- Sequences and classification of group I and group II introns / John M. Burke, James E. Dahlberg and John N. Abelson -- Compilation of self-cleaving sequences from plant virus satellite RNAs and other sources / George Bruening, James E. Dahlberg and John N. Abelson.

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Description based on print version record.

Many methods have been developed or adapted for the study of RNA processing, which plays a key role in the expression of genetic information. Volumes 180 and 181 of Methods in Enzymology, RNA Processing, Parts A and B, represent the first comprehensive collation of protocols used for the preparation of substrates, for the preparation of extracts and enzymes, and for the analysis of products in RNA processing. At least half of the chapters present techniques that are directly applicable to other areas of nucleic acid biochemistry and cell biology.