RNA helicases [electronic resource] / edited by Eckhard Jankowsky.

Includes bibliographical references and indexes.

Analysis of duplex unwinding by RNA helicases using stopped-flow fluorescence spectroscopy / Andrea Putnam and Eckhard Jankowsky -- Analyzing ATP utilization by DEAD-box RNA helicases using kinetic and equilibrium methods / Michael J. Bradley and Enrique M. De La Cruz -- Oxygen isotopic exchange probes of ATP hydrolysis by RNA helicases / David D. Hackney -- Conformational changes of DEAD-box helicases monitored by single molecule fluorescence resonance energy transfer / Alexandra Z. Andreou and Dagmar Klostermeier -- RNA catalysis as a probe for chaperone activity of DEAD-box helicases / Jeffery P. Potratz and Rick Russell -- Molecular mechanics of RNA translocases / Steve C. Ding and Anna Pyle -- Analysis of helicase-RNA interactions using nucelotide analog interference mapping / Annie Schwartz [and others] -- Crystallization and X-ray structure determination of an RNA-dependent hexameric helicase / Nathan D. Thomsen and James M. Berger -- Structural analysis of RNA helicases with small-angle X-ray scattering / Manja A. Behrens .. [et al.] -- Analysis of cofactor effects on RNA helicases / Crystal Young and Katrin Karbstein -- Analysis of DEAD-box proteins in mRNA export / Ben Montpetit, Markus A. Seeliger, and Karsten Weis -- Biochemical characterization of the RNA helicase UPF1 involved in nonsense-mediated mRNA decay / Francesca Fiorini, Fabien Bonneau, and Herv�e Le Hir -- Identification of RNA helicase target sites by UV cross-linking and analysis of cDNA / Markus T. Bohnsack, David Tollervey, and Sander Granneman -- In vivo approaches to dissecting the function of RNA helicases in eukaryotic ribosome assembly / David C. Rawling and Susan J. Baserga -- Analysis of RNA helicases in P-bodies and stress granules / Angela Hilliker -- DEAD-box RNA helicases as transcription cofactors / Frances V. Fuller-Pace and Samantha M. Nicol -- DEAD-box RNA helicases in gram-postive RNA decay / Peter Redder and Patrick Linder -- RNA helicases in cyanobacteria : biochemical and molecular approaches / George W. Owttrim -- Determination of host RNA helicases activity in viral replication / Amit Sharma and Kathleen Boris-Lawrie -- Inhibitors of translation targeting eukaryotic translation initiation factor 4A / Regina Cencic, Gabriela Galicia-V�azquez, and Jerry Pelletier -- Identification and analysis of inhibitors targeting the hepatitis C virus NS3 helicase / Alicia M. Hanson [and others].

This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases. The contributions in this volume cover the broad scope of methods in the research on these enzymes. Several chapters describe quantitative biophysical and biochemical approaches to study molecular mechanisms and conformational changes of RNA helicases. Further chapters cover structural analysis, examination of co-factor effects on several representative examples, and the analysis of cellular functions of select enzymes. Two chapters outline approaches to the analysis of inhibitors that target RNA helicases. This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases The contributions in this volume cover the broad scope of methods in the research on these enzymes.