Methods of adipose tissue biology. Part A [electronic resource] / edited by Ormond A. Macdougald.

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Cover image; Title Page; Table of Contents; Copyright; Contributors; Preface; Chapter One: Adipocyte-Specific Transgenic and Knockout Models; Abstract; 1 Introduction; 2 Loss-of-Function Strategies in Adipocytes In Vivo; 3 Gain-of-Function Strategies in Adipocytes In Vivo; Chapter Two: Imaging White Adipose Tissue with Confocal Microscopy; Abstract; 1 Introduction; 2 Materials for Imaging Adipose Tissue; 3 Methods; 4 Summary; Acknowledgment; Chapter Three: Isolation and Study of Adipocyte Precursors; Abstract; 1 Introduction; 2 Adipose Tissue Depots and Cell Populations.

3 Digestion of Whole WAT for Isolation of SVF4 Flow Cytometry and FACS; 5 Primary Adipocyte Precursor Cell Culture and Differentiation; 6 Digestion of Whole WAT for Isolation of Mature Adipocytes; Acknowledgment; Chapter Four: Imaging of Adipose Tissue; Abstract; 1 Introduction; 2 Imaging of Whole Mounted Adipose Tissue; 3 Imaging of Sectioned Adipose Tissue; Acknowledgment; Chapter Five: Adipose Tissue Angiogenesis Assay; Abstract; 1 Introduction; 2 Materials; 3 Methods; 4 Method Limitations; Acknowledgment; Chapter Six: Quantifying Size and Number of Adipocytes in Adipose Tissue; Abstract.

1 Introduction2 Technical Aspects; 3 Future Challenges and Conclusions; Acknowledgment; Chapter Seven: Use of Osmium Tetroxide Staining with Microcomputerized Tomography to Visualize and Quantify Bone Marrow Adipose Tissue In Vivo; Abstract; 1 Introduction; 2 Materials; 3 Methods; 4 Summary; Acknowledgment; Chapter Eight: Brown Adipose Tissue in Humans; Abstract; 1 Introduction; 2 Positron Emission Tomography; 3 Magnetic Resonance Imaging; 4 Dual Energy Computed Tomography; Chapter Nine: Analyzing the Functions and Structure of the Human Lipodystrophy Protein Seipin; Abstract; 1 Introduction.

2 Technical Aspects3 Discussion; Chapter Ten: Differentiation of Human Pluripotent Stem Cells into Highly Functional Classical Brown Adipocytes; Abstract; 1 Introduction; 2 Experimental Components and Considerations; 3 Notes with Troubleshooting; Chapter Eleven: Analysis and Measurement of the Sympathetic and Sensory Innervation of White and Brown Adipose Tissue; Abstract; 1 Introduction; 2 Surgical Denervation of WAT and BAT Nerves; 3 Chemical Denervation of Adipose Tissue Using 6-Hydroxy-Dopamine; 4 Local Sensory Denervation of Adipose Tissue Using Capsaicin.

5 Assessment of Sympathetic Denervation Using NETO or Content as Measured by HPLC-EC6 Assessment of Sensory Denervation Using CGRP ELIA; 7 Expression of Data; Acknowledgment; Chapter Twelve: Measurement and Manipulation of Human Adipose Tissue Blood Flow Using Xenon Washout Technique and Adipose Tissue Microinfusion; Abstract; 1 Introduction; 2 AT 133Xenon Washout-Principle of the Method; 3 AT Microinfusion-Principle of the Method; 4 Materials; 5 Procedure; 6 Calculations; 7 Other Techniques Used in ATBF Measurement; 8 Conclusion; Acknowledgment.

Chapter Thirteen: Isolation and Quantitation of Adiponectin Higher Order Complexes.

Methods of Adipose Tissue Biology is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers who are world leaders in this field. Detailed lab protocols include methods to visualize adipocytes and adipose tissues in humans and experimental models, converting stem cells into white and brown adipocytes in vitro, evaluating aspects of adipocyte metabolism, inducibly knocking out genes in adipose tissues, and evaluating transcriptional control of adipogenesis on a global scale.