Methods in methane metabolism. Part A, Methanogenesis [electronic resource] / edited by Amy C. Rosenzweig, Stephen W. Ragsdale.

Contributor(s): Rosenzweig, Amy C. (Amy Claire) | Ragsdale, Stephen WMaterial type: Text

TextSeries: Methods in enzymology ; v. 494.Publication details: Amsterdam [Netherlands] ; Boston [Mass.] : Elsevier/Academic Press, 2011. Edition: 1st edDescription: 1 online resource (xlv, 373 p.) : illISBN: 9780123851130 (electronic bk.); 0123851130 (electronic bk.); 9780123851123 (electronic bk.); 0123851122 (electronic bk.)Subject(s): Methanobacteriaceae | Methane -- Metabolism | Methanobacteriaceae | Methane -- metabolism | NATURE -- Plants -- Mushrooms | Methane -- Metabolism | MethanobacteriaceaeGenre/Form: Electronic books.Additional physical formats: Print version:: Methods in methane metabolism. Part A, Methanogenesis.DDC classification: 589.9 LOC classification: QP601 | .M49 v.494ebOnline resources: ScienceDirect | ScienceDirect

Contents:

1. Techniques for cultivating methanogens / Ralph S. Wolfe -- 2. Genetic methods for methanosarcina species / Nicole Buan, Gargi Kulkarni, and William Metcalf -- 3. Genetic systems for hydrogenotrophic methanogens / Felipe Sarmiento B., John A. Leigh, and William B. Whitman -- 4. Molecular tools for investigating ANME community structure and function / Steven J. Hallam ... [et al.] -- 5. Studying gene regulation in methanogenic archaea / Michael Rother, Christian Sattler, and Tilmann Stock -- 6. Growth of methanogens under defined hydrogen conditions / John A. Leigh -- 7. Preparation of [Fe]-hydrogenase from methanogenic archaea / Seigo Shima, Michael Schick, and Haruka Tamura -- 8. Assay of methylotrophic methyltransferases from methanogenic archaea / Donald J. Ferguson, David G. Longstaff, and Joseph A. Krzycki -- 9. Methyl-coenzyme M reductase from methanothermobacter marburgensis / Evert C. Duin, Divya Prakash, and Charlene Brungess -- 10. Methods for analysis of acetyl-CoA synthase : applications to bacterial and archaeal systems / David A. Grahame -- 11. Acetate kinase and phosphotransacetylase / James G. Ferry -- 12. Sodium ion translocation and ATP synthesis in methanogens / Katharina Schlegel and Volker M�uller -- 13. Proton translocation in methanogens / Cornelia Welte and Uwe Deppenmeier -- 14. Measuring isotope fractionation by autotrophic microorganisms and enzymes / Kathleen M. Scott, Gordon Fox, and Peter R. Girguis -- 15. 2-oxoacid metabolism in methanogenic CoM and CoB biosynthesis / David E. Graham -- 16. Biomethanation and its potential / Irini Angelidaki ... [et al.].

Summary: Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field.

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Includes bibliographical references and indexes.

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Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field.

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