Laboratory methods in enzymology : RNA / edited by Jon Lorsch, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Includes bibliographical references and indexes.

Computational prediction of RNA secondary structure / Walter N. Moss -- Reverse-transcription PCR (RT-PCR) / Julia Bachman -- Northern blotting / Shan L. He and Rachel Green -- Explanatory chapter: nuclease protection assays / Elizabeth Eyler -- In vitro transcription from plasmid or PCR-amplified DNA / Julie L. Brunelle and Rachel Green -- Measurement of in vivo RNA synthesis rates / David S. McPheeters and Jo Ann Wise -- Method for measuring mRNA decay rate in Saccharomyces cerevisiae / Wenqian Hu and Jeff Coller -- Analysis of polysomes from bacteria / Daoming Qin and Kurt Fredrick -- Polysome profile analysis : yeast / Martin Posp�isek and Leos Val�asek -- Polysome analysis of mammalian cells / Shan L. He and Rachel Green -- Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae / Wenqian Hu and Jeff Coller -- Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates / Shimyn Slomovic and Gadi Schuster -- Circularized RT-PCR (cRT-PCR) : analysis of the 5' ends, 3' ends, and poly(A) tails of RNA / Shimyn Slomovic and Gadi Schuster -- RNA radiolabeling / Rishi Porecha and Daniel Herschlag -- Fluorescently labeling synthetic RNAs / Max Greenfield and Daniel Herschlag -- Analysis of RNA by analytical polyacrylamide gel electrophoresis / Alexey Petrov, Albet Tsa, and Joseph D. Puglisi -- RNA purification by preparative polyacrylamide gel electrophoresis / Alexey Petrov, Tinghe Wu, Elisabetta Viani Puglisi, and Joseph D. Puglisi -- Explanatory chapter : nucleic acid concentration determination / Sarah Kolitz and Jon Lorsch -- RNA purification : precipitation methods / Sarah E. Walker and Jon Lorsch -- Reverse transcriptase dideoxy sequencing of RNA / Sarah E. Walker and Jon Lorsch -- RNA structure experimental analysis : chemical modification / Zhili Xu and Gloria Culver -- Structural analysis of RNA backbone using in-line probing / Ali Nahvi and Rachel Green.

Description based on print version record.