Immunochemical techniques. Part E, Monoclonal antibodies and general immunoassay methods [electronic resource] / edited by John J. Langone, Helen Van Vunakis.

Contributor(s): Langone, John J. (John Joseph), 1944- | Van Vunakis, Helen, 1924-Material type: Text

TextSeries: Methods in enzymology ; v. 92.Publication details: New York : Academic Press, 1983. Description: 1 online resource (xxiv, 647 p.) : illISBN: 0121819922 (electronic bk.); 9780121819927 (electronic bk.)Other title: Monoclonal antibodies and general immunoassay methodsSubject(s): Monoclonal antibodies | Immunoassay -- Methods | Immunochemistry -- Methods | Immunoassay | Immunochemistry | Monoclonal antibodies | Antibodies, Monoclonal | Immunoassay -- methodsGenre/Form: Electronic books. | Methods.Additional physical formats: Print version:: Immunochemical techniques. Part E, Monoclonal antibodies and general immunoassay methods.DDC classification: 574.19 LOC classification: QP601Online resources: ScienceDirect | ScienceDirect

Incomplete contents:

pts. A-C. [without special title].--pt. D. Selected immunoassays.

Summary: FROM THE PREFACE: Several papers in this volume deal with advances in hybridoma technology and complement the chapter by Galfri and Milstein that appeared in Volume 73 of this series. Methods to enhance the efficiency of producing desired fusion products, to simplify the basic procedures involved, and to assess the specificity and other properties of monoclonal antibodies are discussed. Representative examples illustrate how a battery of monoclonal antibodies with different specificities can be used to study various immunochemical and biochemical problems. Other chapters cover additional procedures that can be used to label antigens and antibodies, separate antigen-antibody complexes, and process data. Some papers classified under one heading or contained in another volume of the series include methods that can be applied in other specific areas. For example, cytotoxicity tests described in Volume 93 may be used to screen supernatant fluids in hybridoma experiments if cytotoxic activity is a desirable property of the monoclonal antibody.

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Includes bibliographical references and indexes.

pts. A-C. [without special title].--pt. D. Selected immunoassays.

Description based on print version record.

FROM THE PREFACE: Several papers in this volume deal with advances in hybridoma technology and complement the chapter by Galfri and Milstein that appeared in Volume 73 of this series. Methods to enhance the efficiency of producing desired fusion products, to simplify the basic procedures involved, and to assess the specificity and other properties of monoclonal antibodies are discussed. Representative examples illustrate how a battery of monoclonal antibodies with different specificities can be used to study various immunochemical and biochemical problems. Other chapters cover additional procedures that can be used to label antigens and antibodies, separate antigen-antibody complexes, and process data. Some papers classified under one heading or contained in another volume of the series include methods that can be applied in other specific areas. For example, cytotoxicity tests described in Volume 93 may be used to screen supernatant fluids in hybridoma experiments if cytotoxic activity is a desirable property of the monoclonal antibody.

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